We narrowed to 18,831 results for: Tec

1. pMRBAD-link-CGFP

   Plasmid

   #52733

   Purpose

   L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   link-CGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JME4472

   Plasmid

   #129658

   Purpose

   URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV_AncBE4max_3xHA

   Plasmid

   #112097

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_3xHA
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET11a-link-NGFP

   Plasmid

   #52732

   Purpose

   IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   link-NGFP
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCTCON2-GFP

   Plasmid

   #158130

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-VCre

   Plasmid

   #89575

   Purpose

   Expresses VCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   VCre recombinase
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHCKan-yibDp-ald*-alaE

   Plasmid

   #134939

   Purpose

   Low phosphate inducible alanine dehydrogenase and alanine exporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   alanine dehydrogenase and L-alanine exporter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DL033:cFos::eGFP-iPAK4

   Plasmid

   #177883

   Purpose

   eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   cFos

   Available Since

   July 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFPL-Rh

   Plasmid

   #61649

   Purpose

   Gateway vector for generating red fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIG3 B

    Plasmid

    #132942

    Purpose

    MLV packaging plasmid for B-tropic MLV gag-pol expression

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    B-tropic MLV gag-pol
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD441_WT_hi

    Plasmid

    #128857

    Purpose

    High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YEp181-CUP1-His-Ubi

    Plasmid

    #99539

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSQT817

    Plasmid

    #53373

    Purpose

    wildtype Cas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pASTA3

    Plasmid

    #24657

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYL322d1/N-eGFP

    Plasmid

    #183151

    Purpose

    Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB2188

    Plasmid

    #67296

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS423-CUP1-His-Ubi

    Plasmid

    #99541

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRBAD-Z-CGFP

    Plasmid

    #52735

    Purpose

    L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    Z+CGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET11a-Z-NGFP

    Plasmid

    #52734

    Purpose

    IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    NGFP+Z-fusion
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-miR-155 toehold switch

    Plasmid

    #184755

    Purpose

    Expresses miR-155 sensor with GFP output

    Depositor

    Allen Liu

    Article

    Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

    Insert

    Green Fluorescence Protein
    

    Use

    Tags

    miR-155 toehold

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28c-Ku

    Plasmid

    #161936

    Purpose

    Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

    Depositor

    Harris Wang

    Article

    Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

    Insert

    ku (mku Mycobacterium tuberculosis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMM117

    Plasmid

    #127212

    Purpose

    BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mEmerald-Vinexin-C-14

    Plasmid

    #54305

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Vinexin (SORBS3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG1238 PINK1-mCherry-FIREtag

    Plasmid

    #205174

    Purpose

    Expresses PINK1-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    PINK1-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRE3G-c-fos-iRFP-B3-repRNA-A

    Plasmid

    #159926

    Purpose

    Timestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SaABEmax

    Plasmid

    #119814

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCoofy64

    Plasmid

    #122016

    Purpose

    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV_ABEmax_3xHA

    Plasmid

    #112098

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCHARGE3

    Plasmid

    #24658

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    Turbo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUCBB-oriT

    Plasmid

    #32556

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLb-Cpf1-pGL3-U6-sgRNA

    Plasmid

    #107682

    Purpose

    Expresses LbCpf1 crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG1244 p65-mCherry-FIREtag

    Plasmid

    #205175

    Purpose

    Expresses p65-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    p65-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAGT10446

    Plasmid

    #192353

    Purpose

    L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-t35S_CGCT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. P16ΔS:S10-(MCS)

    Plasmid

    #108182

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCfB2191

    Plasmid

    #67538

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

    Plasmid

    #48295

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Marathon-RT for Split-PE (pEH59)

    Plasmid

    #190108

    Purpose

    Expresses Marathon-RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCTCON2-BXG

    Plasmid

    #158127

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    mTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge