We narrowed to 7,808 results for: 199

1. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIH-tag RFPt- Rab11a WT

   Plasmid

   #161992

   Purpose

   Retroviral expression vector containing Rab11a tagged with RFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   RAB11A (RAB11A Canis Lupus)
   

   Use

   Retroviral

   Tags

   RFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

   Plasmid

   #161993

   Purpose

   Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R286A Y287A K288A

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBpuro-ErbB3

   Plasmid

   #197362

   Purpose

   Encoding human ErbB3.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   ERBB3 (ERBB3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. COC053

   Plasmid

   #209131

   Purpose

   Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

   Depositor

   Christopher A. O'Callaghan

   Article

   O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

   Insert

   Enzyme BirA (birA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV10.F3

   Plasmid

   #63199

   Purpose

   BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYLsgRNA-AtU3b/LacZ

    Plasmid

    #66199

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    AtU3b

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-C1-INPP4B WT

    Plasmid

    #161995

    Purpose

    Mammalian expression vector containing INPP4B. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP4B (INPP4B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdp43-EGFP construct6

    Plasmid

    #28199

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-314 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pPBpuro-ErbB1

    Plasmid

    #197358

    Purpose

    Encoding human ErbB1.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

    Plasmid

    #221998

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ucp1-empty-miR122

    Plasmid

    #231695

    Purpose

    Empty control vector for pAAV-Ucp1-Cre-miR122

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKUNOC-ErbB1-mScarlet

    Plasmid

    #197360

    Purpose

    Encoding human ErbB1 tagged with mScarlet.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    ERBB1 (EGFR Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pES4/4a

    Plasmid

    #69590

    Purpose

    TCR a chain cDNA in pES4 transgenic construct for creating OT-II mice

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H-2Kb

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Plxna4-AP-His

    Plasmid

    #71999

    Purpose

    Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna4 (Plxna4 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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