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Ecoli PancACe

Plasmid

#215712

Purpose

Expresses acetyl-CoA biosensor in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_TauFRET_3xKQ_wpre

Plasmid

#207522

Purpose

Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

Depositor

Bradley Hyman

Article

Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

Insert

TauFRET_P301L_3xKQ

Use

Lentiviral

Tags

NeonGreen and mTurquoise2

Mutation

P301L; K311Q; K317Q; K321Q

Promoter

EF1a

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-RSET-iGABASnFR2-6xHis

Plasmid

#218885

Purpose

Bacterial expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2

Tags

6xHis

Expression

Bacterial

Mutation

S99A F102Y F104Y L178S

Promoter

T7

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN155(I152L)

Plasmid

#27098

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

bJunVN155(I152L) (Jun and Aequorea Victoria, Rat)

Tags

Myc

Expression

Mammalian

Mutation

I152L in Venus(1-154)

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCX-ThPDE5VV

Plasmid

#134778

Purpose

cGMP FRET biosensor

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

ThPDE5VV

Tags

cpVenus-Venus and mTurquoise2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

Plasmid

#218869

Purpose

Mammalian expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PpipB-GFPLVA

Plasmid

#23344

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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Membrane EGFP

Plasmid

#198056

Purpose

Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

Depositor

Amro Hamdoun

Insert

LCK membrane EGFP (LCK A. victoria, Human, Synthetic)

Use

Sea urchin, mammal, zebrafish

Tags

EGFP

Mutation

Tandem repeat of N-terminal LCK membrane targetin…

Promoter

sp6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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Sender_plasmid2

Plasmid

#140693

Purpose

Encodes inducible expression of RhlI (C4-HSL quorum sensing synthase)

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

RhlI (rhlI )

Tags

ssrA degradation tag (ASV)

Expression

Bacterial

Promoter

engineered lac promoter (IPTG inducible)

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIZ-turq-3xFLAG-TREX1

Plasmid

#164232

Purpose

retroviral plasmid for turq-3xFLAG-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

FLAG and mturquoise

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

Plasmid

#201186

Purpose

Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis

Inserts

PTPN2, transcript variant 1 (PTPN2 Human)
Emerald

Use

Lentiviral

Expression

Mammalian

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5_Ptet

Plasmid

#179336

Purpose

gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMZ3012

Plasmid

#204400

Purpose

A C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator rhlR (rhlR )
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PRhl0L0L12

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_14_GlyOx

Plasmid

#177711

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Sender plasmid

Plasmid

#127677

Purpose

Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

Insert

LuxI

Use

Synthetic Biology

Tags

LVA

Expression

Bacterial

Promoter

pT7

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shTACC3

Plasmid

#59355

Purpose

Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Expression

Mammalian

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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