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NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

4XFLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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ECYFP

Plasmid

#111204

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2001 Feb 9;276(6):3805-10.

Insert

ECFP-Xpress tag-GFP10C fusion

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCSHSP:Cre

Plasmid

#30524

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

Cre recombinase (cre bacteriophage P1)

Use

Cre/Lox; Heat shock inducible

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTYF-2xGfaABC1D-tTA

Plasmid

#19981

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xGfaABC1D-tTA

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-Evf2

Plasmid

#99478

Purpose

Mammalian expression of the Evf2 long non-coding RNA

Depositor

Jhumku Kohtz

Article

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf2

Expression

Mammalian

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGPS21loxFRTNeo

Plasmid

#27180

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R

Use

In vitro transposion

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pRL1058

Plasmid

#70686

Purpose

Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1058

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCas61

Plasmid

#82397

Purpose

IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

LDH

Mutation

V39R

Promoter

cLac143

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149S/F366A

Plasmid

#100165

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149S, F366A

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Article

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNQ071

Plasmid

#242082

Purpose

Expresses E. coli RNAP (gene inserts: rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ)

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ

Tags

His6

Expression

Bacterial

Mutation

ybbR-peptide tag inserted between E15 and E16 in …

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJV170

Plasmid

#180077

Purpose

Backbone to clone MTase genes for expression in TXTL

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Type

Empty backbone

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE

Plasmid

#239727

Purpose

Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH

Plasmid

#239734

Purpose

Plasmid expressing tagBFP with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH

Plasmid

#239735

Purpose

Plasmid expressing tagBFP with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH

Plasmid

#239736

Purpose

Plasmid expressing tagBFP with the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH

Plasmid

#239737

Purpose

Plasmid expressing tagBFP with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2900_pGEEC551_pShip-CAG-tagBFP-bGH

Plasmid

#239738

Purpose

Plasmid expressing tagBFP with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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