We narrowed to 3,519 results for: Braf;

1. p3E-Dnm2b-DN

   Plasmid

   #109574

   Purpose

   Multisite gateway vector for 3' tagging with dominant negative Dynamin2b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Dynamin2b (dnm2b Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   dominant negative

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3E-EHD1b-dEH

   Plasmid

   #109567

   Purpose

   Multisite gateway vector for 3' tagging with EHD1b-delta-EH

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EHD1b (ehd1b Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   delta-EH

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2 Flag Smad3 EPSM A204S

   Plasmid

   #27111

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   T179V S204A S208A S213A; A204S

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

4. Ncerulean-PHdomain

   Plasmid

   #66784

   Purpose

   Expression of PIP2 binding protein in sea urchin embryos

   Depositor

   Amro Hamdoun

   Article

   Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

   Insert

   PLCdelta (PLCD1 Human)
   

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   only contains PH domain

   Promoter

   SP6

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. NmCitrine-PHdomain

   Plasmid

   #66783

   Purpose

   Expression of PIP2 binding protein in sea urchin embryos

   Depositor

   Amro Hamdoun

   Article

   Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

   Insert

   PLCdelta (PLCD1 Human)
   

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   only contains PH domain

   Promoter

   SP6

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. 592 p3e-EST etv2 3’UTR

   Plasmid

   #49003

   Purpose

   Zebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   ets variant gene 2 (etsrp Zebrafish)
   

   Use

   Gateway 3' entry

   Tags

   Expression

   Mutation

   only the 3' UTR

   Promoter

   3' gateway entry vector

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1208 p3e-long etv2 3’UTR

   Plasmid

   #49010

   Purpose

   Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   ets variant gene 2 (etsrp Zebrafish)
   

   Use

   Gateway 3' entry

   Tags

   Expression

   Mutation

   Longest 3' UTR

   Promoter

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tol2-mpx-lyn C466A-GFP

   Plasmid

   #35038

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   Lyn C466A-GFP (lyn Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Tol2-krt4-RFP-miR-223 sponge

   Plasmid

   #97150

   Purpose

   For miR-223 knock down

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   miR-223 sponge
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2 Flag Smad3 AAGY

    Plasmid

    #27004

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    P181A P182A

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

11. p072 pCS xGATA4 + xTRPS1(C119)

    Plasmid

    #11043

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    GATA4 + TRPS1(C119) (trps1, gata4-b Frog)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Fusion construct of XGATA4 (amino acids 1-352) wi…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+-Zeo-miR-430-G-G mm

    Plasmid

    #221728

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-G-G mm
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    G-G mismatch of miR-430 site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid

    #221727

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-10-11 mm
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Mismatch at positions 10 and 11 of miR-430 site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Halo-Tev-Akt3 C119S-2X Flag

    Plasmid

    #87608

    Purpose

    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 protein

    Depositor

    Yimon Aye

    Insert

    AKT3 isoform 2 (AKT3 Human)
    

    Use

    Zebrafish (danio rerio) expression

    Tags

    2 tandem flag tags and His-Halo-Tev

    Expression

    Mammalian

    Mutation

    cysteine 119 residue mutated to serine

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p073 pCS xGATA4tr + xTRPS1(C119)

    Plasmid

    #11044

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    GATA4tr + TRPS1(C119) (trps1, gata4-b Frog)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Fusion construct of XGATA4 (amino acids 1-274) wi…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-15F11-HA-mCh

    Plasmid

    #129591

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-15F11-HA-Halo

    Plasmid

    #129592

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p5e irg1

    Plasmid

    #188698

    Purpose

    Gateway promoter element for the zebrafish irg1 gene

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    irg1 (acod1 Zebrafish)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    irg1

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N1-human lyn(C468A)–GFP

    Plasmid

    #35959

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    c468a

    Promoter

    cmv

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-2E2-HA-mCh

    Plasmid

    #129596

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p026 pCS2-EnR

    Plasmid

    #11028

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    engrailed (en Fly)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Engrailed repressor domain (bases encoding amino …

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST_5xUAS:EB3-mScarlet-I

    Plasmid

    #192358

    Purpose

    EB3 tagged with mScarlet-I, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. neurod1:cre; cmlc2:EGFP

    Plasmid

    #173480

    Purpose

    cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Cre
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2-mCherry-Cenexin

    Plasmid

    #186435

    Purpose

    Expresses mCherry Cenexin in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2+ Zf GFP-vclb

    Plasmid

    #185411

    Purpose

    GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin b (vclb Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2+ Zf cdh1-deltaCyot-GFP

    Plasmid

    #185412

    Purpose

    GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    cdh1 (cdh1 Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain deleted

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SI16-Kit a

    Plasmid

    #58948

    Purpose

    expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

    Depositor

    Gavin Wright

    Article

    Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

    Insert

    Kit receptor a mouse monoclonal antibody (kita Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

34. E1b-GFP-Tol2-Gateway dre SE-zic2a P

    Plasmid

    #89385

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    zic2a (zic2a Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p3E-EHD2b

    Plasmid

    #109569

    Purpose

    Multisite gateway vector for 3' tagging with EHD2b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EHD2b (ehd2 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. E1b-GFP-Tol2-Gateway dre SE-zic2a S

    Plasmid

    #89388

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    zic2a (zic2a Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

37. p3E-Dnm2a-DN

    Plasmid

    #109572

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dynamin2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. kank3_pJet1.2

    Plasmid

    #121981

    Purpose

    for generating kank3 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank3 (kank3 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. kank4_pJet1.2

    Plasmid

    #121982

    Purpose

    for generating kank4 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank4 (kank4 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. delta-LIM Isl1

    Plasmid

    #23261

    Depositor

    Samuel Pfaff

    Article

    Thaler et al Cell. 2002 Jul 26. 110(2):237-49.

    Insert

    Isl1 (Isl1 Rat)
    

    Use

    Avian/xenopus/zebrafish

    Tags

    Lamda-dimer domain

    Expression

    Mammalian

    Mutation

    Delta LIM

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only