Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 16,850 results for: SHE

Showing: 4101 - 4150 of 16850 results

  1. pShuttle-CMV-F2A-T2A

    Plasmid
    #62622
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2A
    Depositor
    Zheng-Xu Wang
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62622-plasmid-map-sequence-id-102955

    Enlarge

  2. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68132-plasmid-map-sequence-id-123472

    Enlarge

  3. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

    Enlarge

  4. pWALIUM10-V5-TurboID-GW

    Plasmid
    #196285
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196285-plasmid-map-sequence-id-384638

    Enlarge

  5. pLenti_PGK_Prkaa1

    Plasmid
    #204356
    Purpose
    Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter
    Depositor
    Günter Schneider
    Insert
    AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    204356-plasmid-map-sequence-id-408696

    Enlarge

  6. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

    Enlarge

  7. pCGN-Sos Cat

    Plasmid
    #32917
    Depositor
    Dafna Bar-Sagi
    Insert
    SOS1 (SOS1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 564-1049
    Promoter
    CMV
    Available since
    Jan. 31, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. pLIX403-ccdB-EBFP2

    Plasmid
    #158562
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158562-plasmid-map-sequence-id-310105

    Enlarge

  10. 2NVR

    Plasmid
    #51340
    Purpose
    Catalytic domain of human histone deacetylase HDAC7
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC7
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pAAV-CAG-Kaede

    Plasmid
    #219433
    Purpose
    AAV expression vector for Kaede
    Depositor
    Yoshiho Ikeuchi
    Insert
    Kaede
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219433-plasmid-map-sequence-id-436974

    Enlarge

  12. pLKO.1-blast shGFP

    Plasmid
    #110419
    Purpose
    shGFP control, silence GFP gene, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA PolIII)
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110419-plasmid-map-sequence-id-227288

    Enlarge

  13. pCDH-EF1-Nluc

    Plasmid
    #73024
    Purpose
    Express Nluc reporter under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73024-plasmid-map-sequence-id-133955

    Enlarge

  14. p3E_he1a:ECFP

    Plasmid
    #113880
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    ECFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    113880-plasmid-map-sequence-id-222569

    Enlarge

  15. pTRE2-Hyg-EGFP

    Plasmid
    #177152
    Purpose
    EGFP for the tet-off system
    Depositor
    Bertrand Collet
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177152-plasmid-map-sequence-id-353296

    Enlarge

  16. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  17. pRV-TdTom-MCS

    Plasmid
    #193711
    Purpose
    Retroviral plasmid DNA for subcloning an oligonucleotide library encoding RNA barcodes
    Depositor
    Colin Akerman
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193711-plasmid-map-sequence-id-387442

    Enlarge

  19. pLIX403-ccdB-EYFP

    Plasmid
    #158564
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158564-plasmid-map-sequence-id-309879

    Enlarge

  20. TP2_crtYB

    Plasmid
    #140233
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    na
    Promoter
    t7 promoter
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    140233-plasmid-map-sequence-id-288147

    Enlarge

  21. pBHt2G-RFP

    Plasmid
    #107162
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Type
    Empty backbone
    Use
    Fungal grobacterium transformation plasmid, teste…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    107162-plasmid-map-sequence-id-205976

    Enlarge

  22. pENTR ARRET

    Plasmid
    #111384
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET
    Use
    Gateway entry cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111384-plasmid-map-sequence-id-209223

    Enlarge

  23. pFastBacMam8_N_Flag_3C

    Plasmid
    #178818
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Use
    Tags
    Flag, 3C
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178818-plasmid-map-sequence-id-351926

    Enlarge

  24. pAAV CCTG 1200x

    Plasmid
    #63088
    Purpose
    AdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    CCUG 1200x
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63088-plasmid-map-sequence-id-104487

    Enlarge

  25. pIC162

    Plasmid
    #63070
    Purpose
    Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    Nuf2 (NUF2 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pColdII-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid
    #75355
    Purpose
    Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376
    Depositor
    Kalle Gehring
    Insert
    Sacsin Xenopus (Silurana) tropicalis 87-1376
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75355-plasmid-map-sequence-id-142888

    Enlarge

  27. pLV-EF1-FLP-PGK-Neo

    Plasmid
    #108544
    Purpose
    Lentivirus vector expessing Flp under EF1a promoter and neomycin under PGK promoter
    Depositor
    Javier Alcudia
    Inserts
    flp recombinase
    Neomycin
    Use
    Tags
    Expression
    Mutation
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108544-plasmid-map-sequence-id-208673

    Enlarge

  28. pCDH-EF1-Fon-tdTomato

    Plasmid
    #72261
    Purpose
    Express tdTomato when FLP is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    elongation factor-1
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72261-plasmid-map-sequence-id-131822

    Enlarge

  30. mEmerald-Hist4HG-N-18

    Plasmid
    #62757
    Purpose
    Localization: Histones, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    Hist4HG (HIST1H4G Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62757-plasmid-map-sequence-id-358367

    Enlarge

  31. pCAGGS-ROSAf

    Plasmid
    #54323
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGG
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54323-plasmid-map-sequence-id-124428

    Enlarge

  32. pcDNA3.3_SARS2_BA.2.87.1

    Plasmid
    #218679
    Purpose
    expressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.87.1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218679-plasmid-map-sequence-id-437265

    Enlarge

  33. pAAV-tMCK-Cyto-TurboID

    Plasmid
    #149411
    Purpose
    Muscle-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection
    Depositor
    Jonathan Long
    Insert
    Cyto-TurboID
    Use
    AAV
    Tags
    V5
    Expression
    Mutation
    Promoter
    tMCK
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149411-plasmid-map-sequence-id-299815

    Enlarge

  34. pFastBac1/MBP-His

    Plasmid
    #38008
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    MBP (Maltose binding protein)
    Use
    Baculovirus expression
    Tags
    6xHIS
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Oct. 1, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pCAGGS-ROSAr

    Plasmid
    #54324
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGG
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54324-plasmid-map-sequence-id-124429

    Enlarge

  36. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92304-plasmid-map-sequence-id-181652

    Enlarge

  37. pLenti_P2A_Blast

    Plasmid
    #211365
    Purpose
    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections
    Depositor
    Jacques Neefjes
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211365-plasmid-map-sequence-id-437557

    Enlarge

  38. mPA-GFP-Lifeact-7

    Plasmid
    #54671
    Purpose
    Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mPA-GFP-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pRSFDuet-galK

    Plasmid
    #193727
    Purpose
    Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK
    Depositor
    Martin Buck
    Insert
    galK (galK E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23113
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193727-plasmid-map-sequence-id-381922

    Enlarge

  40. pLIX403-ccdB-NatMx

    Plasmid
    #158563
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158563-plasmid-map-sequence-id-309878

    Enlarge

  41. pET-His6-Sumo-MBP-CfaN

    Plasmid
    #126016
    Purpose
    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)
    Depositor
    Thomas Muir
    Insert
    His5-Sumo-MBP-CfaN
    Use
    Tags
    5xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126016-plasmid-map-sequence-id-248770

    Enlarge

  42. pBAD-cyScarlet

    Plasmid
    #191260
    Purpose
    Bacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarlet
    Depositor
    Robert Campbell
    Insert
    cyScarlet
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191260-plasmid-map-sequence-id-374869

    Enlarge

  43. pLenti-m5HT6-EGFP

    Plasmid
    #200443
    Purpose
    Lentiviral expression of mouse m5HT6-EGFP
    Depositor
    Ken-Ichi Takemaru
    Insert
    5HT6-EGFP
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200443-plasmid-map-sequence-id-392562

    Enlarge

  44. Flag-TRIM24-F979A/N980A

    Plasmid
    #28142
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    FLAGX2
    Expression
    Mammalian
    Mutation
    F979A and N980A
    Promoter
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  45. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92305-plasmid-map-sequence-id-181650

    Enlarge

  46. pTre-tight-SV40-luciferase (496)

    Plasmid
    #65489
    Purpose
    Inducible expression of coding/noncoding genes
    Depositor
    Rory Johnson, Roderic Guigo
    Insert
    SV40-luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight Tre promoter
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    65489-plasmid-map-sequence-id-158775

    Enlarge

  47. pBV58

    Plasmid
    #204603
    Purpose
    ERG11-3X Minidegron-5X FLAG-HIS3MX6
    Depositor
    Scott Moye-Rowley
    Insert
    Candida glabrata ERG11 (ERG11 )
    Use
    Tags
    auxin-inducible degron
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204603-plasmid-map-sequence-id-403443

    Enlarge

  48. pUAST tdTomato Profilin WT

    Plasmid
    #53509
    Depositor
    Matthew Scott
    Insert
    profilin (chic Fly)
    Use
    Tags
    tdtomato
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid
    #133572
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133572-plasmid-map-sequence-id-274057

    Enlarge

Showing: 4101 - 4150 of 16850 results