We narrowed to 5,538 results for: pcas

1. pCAX APPs-751-alpha

   Plasmid

   #30149

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein - soluble (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   splice variant 751; amino acids 1–612

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCaUra3-CUbo-AID*-GFP

   Plasmid

   #212775

   Purpose

   C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HA-rat Nrxn1beta AS4(-)

   Plasmid

   #58267

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-nDsRedFL-miR9/9*/124

   Plasmid

   #177807

   Purpose

   The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-9/9*/124
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS/XBB.1.5-RBD

   Plasmid

   #213038

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.5-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Gag-FKBP-Myc

   Plasmid

   #177909

   Purpose

   HIV Gag-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   HIV Gag (gag )
   

   Use

   Tags

   FKBP12 and Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9_hGEM(1-110)_hSLBP(56-108)

   Plasmid

   #191388

   Purpose

   SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_hSLBP(56_108)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-SRGAP1FBAR(516a.a.)-EGFP

   Plasmid

   #168505

   Purpose

   Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

   Depositor

   Franck Polleux

   Article

   Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

   Insert

   SRGAP1-FBAR (SRGAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-516

   Promoter

   CAG

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-pac-T2A-dre

   Plasmid

   #124833

   Purpose

   Puromycin resistance gene and dre recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-dre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-opCas12a-Ultra-6xNLS

    Plasmid

    #209024

    Purpose

    Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/M537R/S542R/F870L

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCASP-SptP120-K55-HilA

    Plasmid

    #153329

    Purpose

    Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K55 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-CIBN-EGFP-KRasCT

    Plasmid

    #178527

    Purpose

    Expresses CIBN-EGFP-KRasCT in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CIBN-EGFP-KRasCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-HA-rat Nrxn2beta AS4(-)

    Plasmid

    #58268

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn2beta AS4(-) (Nrxn2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-PB1-AG(Y63L)-LOV2

    Plasmid

    #178876

    Purpose

    Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen(Y63L)-LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AzamiGreen: Y63L

    Promoter

    CAG

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Michael Farzan, Hyeryun Choe

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-Flag-hsDicer (K70A)

    Plasmid

    #41589

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K70A

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9-Puro HLAG-2A-sgRNA

    Plasmid

    #182551

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2A-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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