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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,223 results for: ALP

Showing: 4151 - 4200 of 7223 results

  12. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  13. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

    Plasmid
    #176456
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-L452R-T478K (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L452R, T478K
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176456-plasmid-map-sequence-id-347373

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  14. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid
    #176325
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.7 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176325-plasmid-map-sequence-id-347062

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  18. pVRC8400-SARS-CoV-2-S2P-B.1.351-AVI

    Plasmid
    #176326
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.351 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, 242-244del, R246I, K417N, E484…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176326-plasmid-map-sequence-id-347063

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  21. pVRC8400-SARS-CoV-2-S2P-P.1-AVI

    Plasmid
    #176327
    Purpose
    Expresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-P.1 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176327-plasmid-map-sequence-id-347067

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  26. Fibronectin-human-mutated V1 O-Glycosylation site

    Plasmid
    #120406
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120406-plasmid-map-sequence-id-233165

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  29. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

    Plasmid
    #120407
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with mutations …
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120407-plasmid-map-sequence-id-232461

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  35. 7AA-aSyn-pRK172

    Plasmid
    #236197
    Purpose
    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.
    Depositor
    Yang Yang, Sjors Scheres
    Article
    Yang et al Acta Neuropathol. 2023 May;145(5):561-57
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion os MAAAEKT at residue 22
    Promoter
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236197-plasmid-map-sequence-id-469796

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  44. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

    Plasmid
    #193037
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, L355A, Residues 316-366, Non-native C-term…
    Promoter
    T7
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193037-plasmid-map-sequence-id-379006

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  45. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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Showing: 4151 - 4200 of 7223 results