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Plasmid#199782PurposeSynthetic gene circuitDepositorInsertNO MS-Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NO MS-Sensor (35)
Plasmid#199783PurposeSynthetic gene circuitDepositorInsertNO MS-Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NO- DSD Sensor 1
Plasmid#199785PurposeSynthetic gene circuitDepositorInsertNO- DSD Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBImut-T2A-caMEK
Plasmid#199579PurposeExpress tKiMBImut(AA) and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-Villin
Plasmid#200301PurposeBacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35DepositorInsertbeta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-nsp7
Plasmid#201025PurposeExpresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMV-tet-ONAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1-p75
Plasmid#182945PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTRDepositorUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1
Plasmid#182944PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTRDepositorInsertMNTS-1 (NTF3 Human, Synthetic, Homo sapiens)
UseLentiviralTagsExpressionMammalianMutationThis is the Human NT-3 with mutations on the matu…PromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWP001
Plasmid#178596PurposepAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiTDepositorInsertCBD-SmBiT/LgBiT
UseTagsExpressionBacterialMutationPromoterPxylAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-GBAi W211A
Plasmid#171756PurposeFor the expression of His-GBAi W211A in bacteriaDepositorInsertGBAi
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 1-201-2xStrep
Plasmid#138442PurposeExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsStrepExpressionMammalianMutationAmino acids 1-201PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 1-226-2xStrep
Plasmid#138443PurposeExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsStrepExpressionMammalianMutationAmino acids 1-226PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 1-168-2xStrep
Plasmid#138447PurposeExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids 1-168PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 1-178-2xStrep
Plasmid#138448PurposeExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids 1-178PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 1-203-2xStrep
Plasmid#138449PurposeExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids 1-203PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 1-238-2xStrep
Plasmid#138450PurposeExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids 1-238PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 1-248-2xStrep
Plasmid#138451PurposeExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids 1-248PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 1-273-2xStrep
Plasmid#138452PurposeExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids 1-273PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 1-191-2xStrep
Plasmid#138441PurposeExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsStrepExpressionMammalianMutationAmino acids 1-191PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX-FLAG-CH3
Plasmid#133718PurposeRetroviral expression vector driving CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlagExpressionMutationpartial (not complete) p300 fragment, CH3 domain …PromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-ETS-CORE-MUT
Plasmid#138372PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutatedDepositorInserthuman 161K human enhancer with ETS bind sites mutated
UseGateway compatibleTagsExpressionMutationETS factor binding sites mutatedPromoterNONEAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDK616
Plasmid#134202PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD) mutantDepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationK223A K226APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pIW040
Plasmid#134127PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 R236APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW039
Plasmid#134126PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 and K203A K206APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW028
Plasmid#134125PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 and K183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-MutY-pKK223
Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_19ÎĽ-pcDNA3
Plasmid#111923PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19ÎĽDepositorInsertCeNL(Ca2+)_19ÎĽ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
UseTags6His-TEVExpressionBacterialMutationQ54APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_1.2ÎĽ-pcDNA3
Plasmid#111922PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2ÎĽDepositorInsertCeNL(Ca2+)_1.2ÎĽ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PatoK-OG241
Plasmid#72840PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoL-LOG241
Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
UseTagsExpressionBacterialMutationPromoterPatoLAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoK-LOG241
Plasmid#72848PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-953)
Plasmid#73743PurposeExpression of GFP-PP-RPN2 (918-53) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (932-953)
Plasmid#73745PurposeExpression of GFP-PP-RPN2 (932-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-947)
Plasmid#73747PurposeExpression of GFP-PP-RPN2 (918-947) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-947 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (797-953)
Plasmid#74228Purpose[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM W4108A
Plasmid#83462PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, W4107APromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM S4144D
Plasmid#83460PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, S4144DPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS S4144D-Myc/His
Plasmid#83459PurposeHuman PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4107-4303, S4144DPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His
Plasmid#83457PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148D/F149D)
Plasmid#71388PurposeExpression of hUCH37 (M148D/F149D) in E.coliDepositorInserthUCH37 (ISF1) M148D/F149D (UCHL5 Human)
UseTagsHIS-TEV sequenceExpressionBacterialMutationchanged methionine 148 to aspartate, phenylalanin…PromoterT7Available sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(V41A/W43A)
Plasmid#61402PurposeExpresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cellsDepositorInsertinhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationV41A/W43APromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1529
Plasmid#53027PurposePLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st2), RBS(st3), and glgC leader contains -61 …ExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only