We narrowed to 5,563 results for: SPI

1. pLV-SARS-CoV-2 SL1 alone-Luciferase

   Plasmid

   #191482

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

   Insert

   SARS-CoV-2 5' UTR SL1 alone
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Contains only the stem-loop1 region of SARS-CoV-2…

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST_DHN_AtCor47 (pBS0784)

   Plasmid

   #185254

   Purpose

   For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DHN_AtCor47
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDK197

   Plasmid

   #134189

   Purpose

   Retroviral plasmid expressing LAP-Clasp C terminus

   Depositor

   Iain Cheeseman

   Article

   Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

   Insert

   LAP-Clasp C terminus (CLASP1 Human)
   

   Use

   Retroviral

   Tags

   LAP (GFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Nanog-CreER targeting construct

   Plasmid

   #59720

   Purpose

   Cre-ER knockin targeting construct for mouse endogenous Nanog locus

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   creERT2
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   mNanog

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CKAP2 pHAT

   Plasmid

   #175946

   Purpose

   Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

   Depositor

   Susanne Bechstedt

   Article

   McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

   Insert

   CKAP2 (Ckap2 Mouse)
   

   Use

   Tags

   6-His and StrepTagII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHelA12

   Plasmid

   #176493

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

   Depositor

   Jian-Dong Huang, Xiaodong Cheng

   Article

   Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

   Insert

   SARS-CoV NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   V570I

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus-G protein Gamma 5

   Plasmid

   #42200

   Depositor

   Narasimhan Gautam

   Article

   Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

   Insert

   Gamma 5 (Gng5 Rat)
   

   Use

   Tags

   Venus(1-155)

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 8200 Bicistronic_GFP_ires_hygro

    Plasmid

    #64375

    Purpose

    This a retroviral expression plasmid expressing GFP along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R9-WT

    Plasmid

    #125210

    Purpose

    Expressing wildtype human Rad9b gene

    Depositor

    Richard Finnell

    Article

    Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

    Insert

    Rad9b (RAD9B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

    Plasmid

    #111155

    Purpose

    knock in bait for Axolotl Tyrosinase locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

    Insert

    Tyrosinase-bait-T2a-Cherry-pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid

    #172317

    Purpose

    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJS278

    Plasmid

    #134139

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255, R155A

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

    Plasmid

    #175018

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    OK-HEF s57a ED
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    s57a esterase knock out mutant

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-dLbCpf1-BE0ΔiNLS

    Plasmid

    #107684

    Purpose

    Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0ΔiNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDK480

    Plasmid

    #134197

    Purpose

    Mammalian expression of Flip in SKAP and IRES GFP

    Depositor

    Iain Cheeseman

    Article

    Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

    Insert

    untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Alternate Start, G5 siRNA hardened

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRVH1-hygro shStx3

    Plasmid

    #40071

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Stx3 shRNA-1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCRII-Topo Diap3 in situ probe

    Plasmid

    #45602

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Diap3 in situ probe (Diaph3 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1589-2321 of BC028920

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pCRII-Topo Crim1 in situ probe

    Plasmid

    #45600

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Crim1 in situ probe (Crim1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    insert contains bp#4062-4502 of NM_015800.3

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag

    Plasmid

    #236386

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D156R, D235R, E292R, D350R

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TOPO-SV40

    Plasmid

    #226453

    Purpose

    For subcloning of SV40 promoter or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    SV40
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSF-G1(7CNW)RS

    Plasmid

    #222874

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Tom20-ECFP-FRB

    Plasmid

    #210289

    Purpose

    mitochondrial OM marker and "anchor" in FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-ECFP-FRB
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ECFP-FRB-Sec61b

    Plasmid

    #210290

    Purpose

    nucleus OM marker and "anchor" for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    ECFP-FRB-Sec61b
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Tom20-EYFP-miLID

    Plasmid

    #210291

    Purpose

    mitochondria marker and "anchor" for optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-EYFP-miLID
    

    Use

    Tags

    EYFP and iLID

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mActA-mSspB-mCherry

    Plasmid

    #210294

    Purpose

    ActuAtor for iLID optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    mActA-,SspB-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSF-G1(5CNW)RS

    Plasmid

    #222872

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEMS2075

    Plasmid

    #158098

    Purpose

    High Copy number plasmid containing smCBA insert.

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    smCBA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

    Plasmid

    #194230

    Purpose

    Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB3-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E45D mutation in LCB3

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

    Plasmid

    #194232

    Purpose

    Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-LCB3-PDGFR TMD-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. L2111-FKBP-GPA-20-11S

    Plasmid

    #222882

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. L2375-FKBP-GPA-20-11S

    Plasmid

    #222897

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. L2376-FKBP-GPA-20-114

    Plasmid

    #222896

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. L2374-FRB-GPA-20-11S

    Plasmid

    #222895

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. L2373-FRB-GPA-20-114

    Plasmid

    #222894

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. L2179-FRB-GPA-6-GFPS

    Plasmid

    #222893

    Purpose

    Biosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-6GS-GFPS
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. L2092-GPA-FullBRET-Retrovirus-TD142

    Plasmid

    #222877

    Purpose

    Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-NanoLuc-CyOFP1
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMXS-IRES-Blast coUCK2 D62A

    Plasmid

    #211530

    Purpose

    Expresses codon optimized catalytic mutant UCK2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UCK2 D62A (UCK2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D62A (catalytic mutant)

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. L2110-FKBP-GPA-20-114

    Plasmid

    #222881

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. L2108-FRB-GPA-20-114

    Plasmid

    #222879

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. L2109-FRB-GPA-20-11S

    Plasmid

    #222880

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBiFC-VN155(I152L)-SRCR5

    Plasmid

    #207993

    Purpose

    BiFC assay

    Depositor

    Young Tang

    Article

    Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

    Insert

    SRCR5 (CD163 pig)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA5-FRT-TO-nsp7

    Plasmid

    #201025

    Purpose

    Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet-ON

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pYPQ230-D156R

    Plasmid

    #188542

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r entry c…

    Tags

    NLS

    Expression

    Plant

    Mutation

    D156R

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. DsRed -7e

    Plasmid

    #191765

    Purpose

    Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Use

    Tags

    N terminal fusion of negatively charged polypetod…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

    Plasmid

    #191481

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR ΔSL1
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA 3.1 BS (-) N protein

    Plasmid

    #188448

    Purpose

    SARS CoV-2 N protein expression

    Depositor

    Ajit Chande

    Article

    Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

    Insert

    SARS-CoV-2 N protein
    

    Use

    Tags

    Strep-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pST_CAHS1_PARRC_150-189 (pBS0658)

    Plasmid

    #185201

    Purpose

    For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS1_PARRC_150-189
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge