We narrowed to 30,996 results for: uros

1. LentiU6-LacZ-GFP-Puro (BB)

   Plasmid

   #170459

   Purpose

   For cloning gRNA block

   Depositor

   Yonglun Luo

   Article

   Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

   Insert

   hU6-BB-LacZ and EF1A-EGFP-2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 for gRNA and EF1A for EGFP-2A-Puro

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Puro-cRAF-W22

   Plasmid

   #15270

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   CRAF (RAF1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   C-terminal deletion

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pLIX403-EBFP2-ccdB-Puro

   Plasmid

   #158552

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTet-O-E2F7-T2A-PuroR

   Plasmid

   #162348

   Purpose

   Lentiviral expression of E2F7 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL6-H2B-iRFP720-puro

   Plasmid

   #189992

   Purpose

   Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   Human histone H2B
   

   Use

   Lentiviral

   Tags

   iRFP720

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. OCT4 GFP puro Donor 1

   Plasmid

   #22209

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-HIF2α P531A-pBabe-puro

   Plasmid

   #26091

   Depositor

   William Kaelin

   Article

   Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA with point mutation P531A

   Promoter

   Available since

   Sept. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. plex_307-3xflag-ccdb-puro

   Plasmid

   #201122

   Purpose

   pLEX_307 plasmid with N-terminal FLAG tag and puro marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAuroraA GFP-AURKA-GFP

    Plasmid

    #157765

    Purpose

    Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    promoter CMV replaced by AurkA promoter

    Promoter

    AURKA minimal

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-PtenC124S-Puro

    Plasmid

    #135677

    Purpose

    Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-U6sgbbBsmbI-puro-2A-Fluc

    Plasmid

    #100277

    Purpose

    Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe puro H-Ras 61L

    Plasmid

    #13485

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    HRas 61L (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    61L

    Promoter

    Available since

    Dec. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pBabe puro RalA Q75L

    Plasmid

    #19719

    Depositor

    Channing Der

    Article

    Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

    Insert

    V-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    GTPase deficient & constitutively active Gl…

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXspuro-GFP-NBR1-dLIR

    Plasmid

    #74204

    Purpose

    Retroviral expression of GFP-NBR1 with aa 727-738 deletion

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    neighbor of BRCA1 gene 1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 727-738

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

18. PITX3-eGFP-PGK-Puro

    Plasmid

    #31942

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pALPS puro miR30-L1221

    Plasmid

    #101335

    Purpose

    negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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