We narrowed to 13,713 results for: TIM

1. pcDNA3.2/NS4A-2K-NS4B

   Plasmid

   #120828

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS4A-2K-NS4B ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM6-FAS

   Plasmid

   #49810

   Purpose

   Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   fatty acyl-ACP thioesterase
   hydroxydecanoyl-ACP dehydratase
   b-ketoacyl-ACP synthase III
   3-ketoacyl-ACP reductase
   enoyl-(acyl carrier protein) reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon-optmized. See supplemental information of …

   Promoter

   T7

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-FOXO3a WT DBM (H212R)

   Plasmid

   #8352

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H212R

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCRU5-inmCherry

   Plasmid

   #60238

   Purpose

   HTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intron

   Depositor

   Dmitriy Mazurov

   Article

   Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

   Insert

   inmCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiEKAR2G2

   Plasmid

   #40178

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSws1-nfsB-CFP

   Plasmid

   #86375

   Purpose

   for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

   Depositor

   Rachel Wong

   Article

   Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

   Insert

   nitroreductase (nfsB bacteria)
   

   Use

   Zebrafish

   Tags

   ECFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA flag-Pcbp2

   Plasmid

   #184028

   Purpose

   Codon optimized Pcbp2 clone with N-terminal flag tag

   Depositor

   Peter Stoilov

   Article

   Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

   Insert

   Pcbp2 (Pcbp2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

   Plasmid

   #171795

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-kanMX6-Purg1-3HA

    Plasmid

    #39351

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA and kanMX6 module - reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pShuttle

    Plasmid

    #192871

    Purpose

    Gateway compatible vector with BsaI cloning site

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Orange Nano-lantern/pcDNA3

    Plasmid

    #67661

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. Cyan Nano-lantern/pcDNA3

    Plasmid

    #67660

    Purpose

    cyan luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Cyan Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGTag-P2A-QFGal4-SV40pA

    Plasmid

    #155127

    Purpose

    Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    P2A-QFGal4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR3.1 GSK3beta S9A HA

    Plasmid

    #15994

    Depositor

    Morris Birnbaum

    Article

    Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

    Insert

    GSK3beta (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A: constitutively active

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

16. RT3GEN

    Plasmid

    #111165

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA5/FRT/TO_H2B_HaloTag9

    Plasmid

    #169334

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNXgate32-3HA

    Plasmid

    #105076

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    3xHA and NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3E-ING2

    Plasmid

    #109554

    Purpose

    Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiEKAR2G1

    Plasmid

    #40177

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a(TIGIT)

    Plasmid

    #177902

    Purpose

    Bacterial expression of his10-tagged human TIGIT Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    TIGIT (aa 163-244 only) (TIGIT Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMMK ENTR 1

    Plasmid

    #206260

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 1

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMMK ENTR 3

    Plasmid

    #206262

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 3

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMMK ENTR 4

    Plasmid

    #206263

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 4

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFLEX-m-cyclin D1 -T286A mutant

    Plasmid

    #86792

    Purpose

    mammalian expression of cyclin D1 T286A mutant

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D1 (Ccnd1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T286A

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHis-zfCSF3b

    Plasmid

    #65613

    Purpose

    express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3b (csf3b Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1 shTGFBR3 puro

    Plasmid

    #58696

    Purpose

    Lentiviral shRNA vector for knockdown of human TGFBR3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shTGFBR3 (TGFBR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

29. LT3REVIN

    Plasmid

    #111175

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSR02

    Plasmid

    #69149

    Purpose

    Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-DUET-GST-eIF4G1-His6, eIF4E

    Plasmid

    #37232

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Inserts

    TIF4631 (TIF4631 Budding Yeast)
    CDC33 (CDC33 Budding Yeast)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pJH4687

    Plasmid

    #200783

    Purpose

    Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    ins-22 (ins-22 Nematode)
    

    Use

    Tags

    pHiuorin

    Expression

    Worm

    Mutation

    Promoter

    Pnpr-4

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pPMVAd36

    Plasmid

    #98302

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRZ-mNanog-YNL

    Plasmid

    #65717

    Purpose

    mNanog reporter plasmid (expresses YNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Nanog promoter and YNL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pROF394

    Plasmid

    #155333

    Purpose

    Expression of PULSE-controlled LFY-VP16-2A-RLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV5 Flag-Smad2 EPSM

    Plasmid

    #14933

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

    Insert

    Smad2 EPSM (SMAD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Erk/Pro-directed kinase site mutant (EPSM). T220V…

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pXNgate21-3HA

    Plasmid

    #105078

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CytERM-mTurquoise2

    Plasmid

    #98833

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    CytERM
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge