We narrowed to 5,867 results for: nls

1. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWL078 MCP-2xGFP

   Plasmid

   #197047

   Purpose

   pCYC-NLS-MCP-2xGFP on pRS305

   Depositor

   Robert Singer

   Article

   Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

   Insert

   MCP-2xGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ11440

   Plasmid

   #183203

   Purpose

   pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE

   Depositor

   Stanley Qi

   Article

   Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

   Insert

   hyperdCas12 base editor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNSEN-d2

   Plasmid

   #59763

   Purpose

   Expresses nuclear EGFP and Tomato in mammalian cells

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

   Inserts

   d2-EGFP
   Tomato
   

   Use

   Tags

   3X NLS and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter and SV40 promoter

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BCJJ125

   Plasmid

   #117515

   Purpose

   Cas9_3 (with intron) Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_3(intron):GCTT:BsaI
   

   Use

   CRISPR

   Tags

   2xFLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKF295

   Plasmid

   #72871

   Purpose

   expression of improved FLP recombinase

   Depositor

   Klaus Foerstemann

   Article

   Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

   Insert

   FLP recombinase (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   P2->S, added NLS

   Promoter

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EZ-L560

   Plasmid

   #173028

   Purpose

   Encodes OptoAMP2 circuit

   Depositor

   Jose Avalos

   Article

   Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo

   Plasmid

   #62345

   Purpose

   Expresses MCP-tdiRFP670 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

   Insert

   MCP-tdiRFP670
   

   Use

   Tags

   HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EZ-L545

    Plasmid

    #173024

    Purpose

    Encodes OptoEXP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCNPpo6

    Plasmid

    #46086

    Depositor

    Raymond Monnat

    Article

    Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

    Insert

    PpoI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EZ-L580

    Plasmid

    #173023

    Purpose

    Encodes OptoAMP4 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXS-IRES-BLAST co hPHGDH

    Plasmid

    #154915

    Purpose

    Express codon optimized human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH, codon optimized (PHGDH Synthetic, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOG231

    Plasmid

    #17736

    Depositor

    Geoff Wahl

    Article

    O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

    Insert

    NLS-Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCNCre3

    Plasmid

    #46088

    Depositor

    Raymond Monnat

    Article

    Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

    Insert

    CreI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNuc-TrxRFP1

    Plasmid

    #98998

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and nuclear localization sequence
    

    Use

    Tags

    His-tag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKI1.1

    Plasmid

    #85807

    Purpose

    CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. act-AsCpf1

    Plasmid

    #73917

    Purpose

    expression plasmid of AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    hAsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EZ-L175

    Plasmid

    #173025

    Purpose

    Encodes OptoAMP1 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRETightBI-RY-HMGA2wt

    Plasmid

    #31469

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pBSKΔB-TRE-dCas9-EGFP

    Plasmid

    #62347

    Purpose

    Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    dCas9-EGFP
    

    Use

    CRISPR

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIR190

    Plasmid

    #64812

    Purpose

    TCP4 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al PLoS Genet. 2013 Mar;9(3):e1003374. doi:

    Insert

    TCP4 (TCP4 Mustard Weed)
    

    Use

    Tags

    GAL4 Activation Domain and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNXRVj-HACre

    Plasmid

    #78407

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    aur1R (aur1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Gly 240 to Cys

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RHD_exon1_TALEN_R

    Plasmid

    #62240

    Purpose

    expression of TALEN targeting human RHD exon1

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon1_TALEN_Right
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RHD_exon1_TALEN_L

    Plasmid

    #62239

    Purpose

    expression of TALEN targeting human RHD exon1

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon1_TALEN_Left
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BCJJ180

    Plasmid

    #117516

    Purpose

    Cas9_4 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_4:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTRETightBI-RY-4

    Plasmid

    #31465

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

28. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC2HB

    Plasmid

    #63734

    Purpose

    bait plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans bait plasmid

    Tags

    HA, LexA, and NLS

    Expression

    Mutation

    Promoter

    Met3

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRETightBI-RY-1

    Plasmid

    #31464

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pC2HP

    Plasmid

    #63735

    Purpose

    prey plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans prey plasmid

    Tags

    FLAG, NLS, and VP domain

    Expression

    Mutation

    Promoter

    Met3

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTSK368

    Plasmid

    #34551

    Depositor

    James McNally

    Article

    Karpova et al Science. 2008 Jan 25;319(5862):466-9.

    Insert

    MS2-CP-NLS
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTRETightBI-RY-7

    Plasmid

    #31466

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pTRETightBI-RY-1pf

    Plasmid

    #31467

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTRETightBI-RY-HMGA2mutant

    Plasmid

    #31470

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pYZ675

    Plasmid

    #132954

    Purpose

    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    adh1

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 6xHis-MBP-huPcCas12a

    Plasmid

    #174681

    Purpose

    Expresses 6xHis-MBP tagged PcCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPcCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 6xHis-MBP-huCMtCas12a

    Plasmid

    #174680

    Purpose

    Expresses 6xHis-MBP tagged CMtCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMtCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 6xHis-MBP-huPdCas12a

    Plasmid

    #174682

    Purpose

    Expresses 6xHis-MBP tagged PdCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPdCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge