161,570 results

1. pCI HA NEDD4

   Plasmid

   #27002

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   NEDD4 (NEDD4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-Rab11a-7

   Plasmid

   #55124

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab11a (RAB11A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+MLS-HyPer7

   Plasmid

   #136470

   Purpose

   Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Tandem mitochondrial targeting signal of cytochro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pED9x (dCas9-KRAB-mCherry)

   Plasmid

   #163956

   Purpose

   Lentiviral expression plasmid of sgRNA with mCherry

   Depositor

   Junwei Shi

   Article

   Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. gag-PE

   Plasmid

   #211371

   Purpose

   Packaging plasmid for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-PE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-3FLAG-EEA1

   Plasmid

   #176491

   Purpose

   For lentiviral transduction of 3FLAG-EEA1

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-gp32-H6

   Plasmid

   #163912

   Purpose

   Expresses T4 gp32 for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   gp32
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AiP12558 - pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2558)

   Plasmid

   #208141

   Purpose

   AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T MDM2 WT

   Plasmid

   #16237

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.0-TERT WT

    Plasmid

    #84924

    Purpose

    luciferase reporter for TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CHOP promoter (-649/+136) pmCherry-1

    Plasmid

    #36035

    Depositor

    Quan Lu

    Article

    Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

    Insert

    CHOP promoter (-647 to +136) (DDIT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBbi-GP

    Plasmid

    #60511

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_BiPVe4_dTomato_nlsdTomato

    Plasmid

    #213936

    Purpose

    AAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    dTomato_P2A_nls.dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Use

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLminP_Luc2P_RE17

    Plasmid

    #90359

    Purpose

    NFAT - PKC/Ca++ gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-GRAB_r5-HT1.0

    Plasmid

    #208719

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge