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Plasmid#211853PurposeE3-DART degron casette for N-terminal fusion to protein of interest using GoldenbraidDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAllet
Plasmid#202560PurposeE. coli cloning vector with large MCS (50+ unique restriction sites)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
DinoIII
Plasmid#128100PurposeDinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.DepositorInsertRNA complex & upstream/downstream of LHC
UseTagsExpressionBacterialMutationPromoterInserts are potential promotersAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
BsaI-tagRFP-BsaI-pUC-kan
Plasmid#170771Purposetest plasmid for Golden Gate assembly, encodes fluorescent red proteinDepositorInserttagRFP
UseTagsExpressionMutationPromoternoneAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(Trp1)
Plasmid#49459Purposeoverespression of Flp step-arrest mutant in yeast, TRP1 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pID3.01B
Plasmid#22720DepositorInsertunc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCBLggCDS
Plasmid#199737PurposeGolden Gate entry vector for gene of interest cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) TRIM12C
Plasmid#87080PurposeMurine homologues of human Trim5DepositorInsertTRIM12C (Trim12c Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) TRIM12A
Plasmid#87061PurposeMurine homologues of human Trim5DepositorInsertTRIM12A (Trim12a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDL1403
Plasmid#226635PurposeThis plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.DepositorInsertstfox
qstR
UseTagsExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDL1531
Plasmid#226637PurposeThis plasmid is for correcting the nonsense mutation in the nrsB gene of several ICP1 isolates.DepositorInsertUpstream sequence of nrsB (only 338bp total)
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAT
Plasmid#112587PurposeUsed for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequenceDepositorTypeEmpty backboneUseTags6His-GSTExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) TRIM30B
Plasmid#87082PurposeMurine homologues of human Trim5DepositorInsertTRIM30B (Trim30b Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-PH(Btk)KD(Akt1)
Plasmid#86636PurposeExpresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1DepositorInsertPH(Btk)KD(Akt1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUA
Plasmid#58372PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)61-cpGFP-ERexp
Plasmid#195311PurposeFluorescent reporter/biosensor for membrane tensionDepositorInsertMscL(G22S)61-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRL093 pattB_exorh:EGFP_MCS
Plasmid#213514PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACUH
Plasmid#58374PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
290 RFP/CFP
Plasmid#83322Purposefluorescent coding joint substrateDepositorInsertRFP +CFP joining substrate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL092 pattB_cryaa:Venus_MCS
Plasmid#213513PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only