We narrowed to 9,416 results for: Coli
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Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-lacZ
Plasmid#72953PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-RFP
Plasmid#72957PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-PhCHS-MsCHI
Plasmid#73400PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
PhCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-CmCHS-CmCHI
Plasmid#73403PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
CmCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-CmCHS-CmCHI
Plasmid#73399PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
CmCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_EF
Plasmid#66060PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_AE
Plasmid#66065PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_EF
Plasmid#66066PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_AE
Plasmid#66057PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66058PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_AE
Plasmid#66059PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_AB
Plasmid#66016PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_EB
Plasmid#66017PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_FB
Plasmid#66018PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_GB
Plasmid#66019PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
B0033m_BC
Plasmid#66021PurposeMoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
B0034m_BC
Plasmid#66022PurposeMoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0080 (araC)_CD
Plasmid#66029PurposeMoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]DepositorInsertTranscription factor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZS1[Iq:LacI-T]
Plasmid#60756PurposeContains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.DepositorInsertLacI-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI without the tetramerization domain and the T…PromoterAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1035
Plasmid#52999PurposepLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
fhlA
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1033
Plasmid#52998PurposepLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
rpoS
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −150 and +30 bp relative to start codon …PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa02_2817
Plasmid#49654DepositorInsertECF02_2817
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ptef1-CSC-Tpdc1
Plasmid#127739PurposeYeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available SinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Pzuo1-CSC-Tvma16
Plasmid#127740PurposeYeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPzuo1Available SinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ppfy1-CSC-Taip1
Plasmid#127741PurposeYeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpfy1Available SinceAvailabilityAcademic Institutions and Nonprofits only -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOP3SU
Plasmid#112599PurposeUsed for expression of protein in E. coli as N-terminal Sumo tag fusionsDepositorTypeEmpty backboneUseTagsSumoExpressionBacterialMutationPromoterAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpot2
Plasmid#117063PurposeFor expression of proteins with C-terminal Spot-Tag in E. coliDepositorTypeEmpty backboneUseTagsSpot-TagExpressionBacterialMutationPromoterAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP2H
Plasmid#112596PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusionsDepositorTypeEmpty backboneUseTags8HisExpressionBacterialMutationPromoterAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTar-ipgC
Plasmid#97097PurposeExpresses ipgC in the presence of E. coli sigma factor ?28DepositorInsertIpgC
UseTagsExpressionBacterialMutationPromoterpTarAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest-557
Plasmid#161890PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coliDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mcherry2
Plasmid#87145PurposeConstitutive E. coli mcherry Expression, with DAS4 tagDepositorInsertmcherry
UseTagsC terminal DAS4 tagExpressionBacterialMutationPromoterConstitutive proDAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOP2S
Plasmid#112597PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusionsDepositorTypeEmpty backboneUseTagsStrep tagExpressionBacterialMutationPromoterAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAMHDAdGT8-4
Plasmid#26421DepositorInsertHSV-TK, mCMV-b-gal
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIHDAdGT8-3
Plasmid#26525DepositorInsertHSV-TK, mCMV-b-gal
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable SinceMay 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTar-deGFP
Plasmid#97098PurposeExpresses GFP in the presence of E. coli sigma factor ?28DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterpTarAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
UseTagsExpressionBacterialMutationPromoterSpectinomycin resistance gene promoter and noAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
UseTagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialMutationPromoterT7Available SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn
Plasmid#100947PurposeExpression of Tyrosine Ammonia Lyase from inducible T7 promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-mNeongreen
Plasmid#182389PurposeBacterial expression of Vesicle Nucleating peptide15-mNeongreen fusionDepositorInsertVNp15-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedTagsExpressionMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
CTV
Plasmid#15912DepositorInsertCAG-STOP-eGFP-ROSA26TV
UseCre/Lox; /stopTagsExpressionMutationPromoterAvailable SinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_DNAseI
Plasmid#182408PurposeBacterial expression of DNaseIDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C3
Plasmid#189620PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRemoved repeat region, 10-kbp deletion, and minor…PromoterAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2355_Tier3(PB)-iRFP_PuroR
Plasmid#169654PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only