We narrowed to 8,206 results for: Dos
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Plasmid#110883PurposePmec-4 Gateway Destination vector for cloning for expression in C. elegans mechanosensory neuronsDepositorInsertPmec-4
UseGateway CloningAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-GLICNot
Plasmid#140182PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and NotI siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsGSTPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)
Plasmid#134332PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA16_Lmo
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)
Plasmid#134334PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA17_Sga
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)
Plasmid#134335PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA18_Sma
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)
Plasmid#134336PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA19_Ssim
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)
Plasmid#134338PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA18_Sga
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)
Plasmid#134339PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA19_Spse
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH2256
Plasmid#132365PurposePrgef-1 INS-6::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-6::GFPDepositorAvailable SinceJan. 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2933
Plasmid#132363PurposePrgef-1 INS-3::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-3::GFPDepositorAvailable SinceDec. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH1922
Plasmid#132361PurposePrgef-1 INS-1::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-1::GFPDepositorAvailable SinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2255
Plasmid#132364PurposePrgef-1 INS-4::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-4::GFPDepositorAvailable SinceOct. 7, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2947
Plasmid#132362PurposePrgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFPDepositorAvailable SinceOct. 7, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFGL1010
Plasmid#119081PurposeIntroducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).DepositorTypeEmpty backboneUseFungal expression (in magnaporthe oryzae)Available SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17
Plasmid#121386PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC92
Plasmid#118910Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagseGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z globin NB Probe
Plasmid#120390PurposeAllows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probeDepositorInsertbeta globin partial 3' UTR
UseUnspecifiedAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPI461
Plasmid#113215Purposegeneric knockin vector for C-terminal tagging with mCherryDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsmCherryAvailable SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC207
Plasmid#118869Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherryDepositorInsertNcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mCherryExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC81
Plasmid#118900Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GSTDepositorInsertNcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease; GST tagExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC215
Plasmid#118879Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
TagsDendra2, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0039
Plasmid#117606PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)DepositorInsert[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0011
Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-xlH2B(1-114)_K114A
Plasmid#118217PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.DepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialMutationLysine 114 to alaninePromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA12
Plasmid#113663PurposeCloning vector for bacterial expression with Pdhfr promoterDepositorTypeEmpty backboneExpressionBacterialPromoterPdhfrAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI184
Plasmid#113221Purposegeneric knockin vector for C-terminal tagging with mCherryDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsmCherryAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI192
Plasmid#113219Purposegeneric knockin vector for C-terminal tagging with mCherryDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsmCherryAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VCL_Vinculin
Plasmid#110045PurposeProtein expression and purification of VCL_VinculinDepositorInsertVCL_Vinculin (VCL Human)
ExpressionBacterialAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-GFP-Psc-StrepII
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
TagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PfSSB_W166C
Plasmid#103001PurposeThe expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteineDepositorInsertPfSSB_W166C
ExpressionBacterialMutationC93A, W166CPromoterT7Available SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-T830E
Plasmid#91996PurposeExpresses FLAG-HA-AGO2 (T830E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S834E
Plasmid#91998PurposeExpresses FLAG-HA-AGO2 (S834E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-S828A
Plasmid#91982PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824A
Plasmid#91987PurposeExpresses FLAG-HA-AGO2 (S824A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-T830A
Plasmid#91988PurposeExpresses FLAG-HA-AGO2 (T830A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S831A
Plasmid#91989PurposeExpresses FLAG-HA-AGO2 (S831A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S834A
Plasmid#91990PurposeExpresses FLAG-HA-AGO2 (S834A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828A
Plasmid#91991PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E
Plasmid#91994PurposeExpresses FLAG-HA-AGO2 (S824E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828E
Plasmid#91995PurposeExpresses FLAG-HA-AGO2 (S828E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A3-T
Plasmid#92019PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N-terminal TEV cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom4.2
Plasmid#87347PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 4.2 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter4.2 kbp proximal promoterAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom0.6
Plasmid#87353PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 0.6 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter0.6 kbp proximal promoter of human MCT2 (nested d…Available SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Stx3 triple mutant (F31A D33A E34A)
Plasmid#86945PurposeExpresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)DepositorInsertSTX3 triple mutant (F31A D33A E34A) (STX3 Human)
Tagsmyc-His6ExpressionMammalianMutationtriple mutant (F31A D33A E34A)Available SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_GB
Plasmid#78669Purpose36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_EB
Plasmid#78667Purpose36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB419
Plasmid#68684PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB134
Plasmid#68588PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGlucocorticoid receptor (GR)ExpressionPlantPromoterMpHSP17.8A1 promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only