We narrowed to 34,407 results for: CaS;

1. pPIK16045_pX330_Flag-SuperFi-Cas9

   Plasmid

   #184370

   Purpose

   Mammalian expression of SuperFi-Cas9 nuclease

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

   Promoter

   CBh

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-3xFlag-dCas13-mTET2HxDCD

   Plasmid

   #228235

   Purpose

   For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

   Depositor

   Chuan He

   Article

   Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

   Inserts

   dCas13
   TET2 (Tet2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Promoter

   CAG

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRubiC-T2A-Cas9

   Plasmid

   #75347

   Purpose

   Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   mCherry (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCerulean3-mVenus-CASPER

   Plasmid

   #58162

   Purpose

   Localization: Cytosolic FRET, Excitation: , Emission:

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mCerulean3 and mVenus-CASPER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET23b-Casp9-His

   Plasmid

   #11829

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

   Insert

   Caspase 9 (CASP9 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pIVT-dCas9-mCherry

   Plasmid

   #174443

   Purpose

   in vitro transcription template for dCas9-mCherry

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Cas9 Endonuclease Dead
   

   Use

   In vitro transcription template

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   T7 SP6

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-SpdCas9-VP64

   Plasmid

   #115794

   Purpose

   Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   VP64
   

   Use

   AAV

   Tags

   3XFLAG and dCas9-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-UltraCas12a-6xNLS

   Plasmid

   #209023

   Purpose

   Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   UltraCas12a-6xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   M537R/F870L

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-EF1a-Cas9GFP-W

    Plasmid

    #200100

    Purpose

    Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRha-ABE8e-SpCas9-NG

    Plasmid

    #201190

    Purpose

    Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

    Depositor

    Jonathan Yen

    Article

    Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

    Insert

    ABE8e-SpCas9-NG
    

    Use

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113343

    Purpose

    Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    p300core-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Casp2-Flag

    Plasmid

    #11811

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 (CASP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lv EF1a dCas9-HA 2A Blast

    Plasmid

    #102812

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    dCas9-HA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A (dCas9)

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRISPR-SP-Cas9 reporter

    Plasmid

    #62733

    Purpose

    Mammalian CRISPR-SP-Cas9 reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
    

    Use

    CRISPR and Lentiviral

    Tags

    CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJet1.2 kanR cassette

    Plasmid

    #117122

    Purpose

    Kanamycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Kanamycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-dCasRx-ccdB

    Plasmid

    #221003

    Purpose

    Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSU-araC-Cas9

    Plasmid

    #120423

    Purpose

    Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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