We narrowed to 39,949 results for: ANT

1. pDGE669

   Plasmid

   #153235

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE672

   Plasmid

   #153238

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265H

   Plasmid

   #149554

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   hAID-ziSpyMacCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ266B

   Plasmid

   #149555

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   ziSpyMacCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ150-x3.7

   Plasmid

   #124308

   Purpose

   Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Insert

   pcoCas9-x3.7
   

   Use

   CRISPR

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBQ10:sXVE:ATG-3xHA-S11

   Plasmid

   #108238

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Insert

   ATG-3xHA-S11
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBRC169-DXMT

   Plasmid

   #79822

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaDXMT1
   

   Use

   Reporter plasmid

   Tags

   RC169/DsREDmonomer (169–225)

   Expression

   Plant

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBRN168-DXMT

   Plasmid

   #79821

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaDXMT1
   

   Use

   Reporter plasmid

   Tags

   RN168/DsREDmonomer (1–168)

   Expression

   Plant

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBRC169-MXMT

   Plasmid

   #79820

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaMXMT1
   

   Use

   Reporter plasmid

   Tags

   RC169/DsREDmonomer (169–225)

   Expression

   Plant

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

    Plasmid

    #68213

    Purpose

    Transcriptional unit for Hygromycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hyg
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SI13-Lrrc3b

    Plasmid

    #46303

    Depositor

    Gavin Wright

    Article

    Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

    Insert

    Lrrc3b mouse monoclonal antibody (low affinity) (lrrc3b Zebrafish)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pYPQ-Cas9-Act3.0

    Plasmid

    #178954

    Purpose

    It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ-dzCas9-Act3.0

    Plasmid

    #158414

    Purpose

    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid

    #68214

    Purpose

    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 silencing suppressor
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB161 - pL2_pSB90_2x35S::GUS::tMAS

    Plasmid

    #123197

    Purpose

    binary plant vector for transient expression of GUS (with introns)

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::GUS::tMAS
    

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMP71-super-IL-2

    Plasmid

    #174599

    Purpose

    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCP60 RS-GFP

    Plasmid

    #122173

    Purpose

    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

    Depositor

    Lukas Fischer

    Article

    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

    Insert

    RS-GFP
    

    Use

    Binary vector for escherichia coli and agrobacter…

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211845

    Purpose

    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Inserts

    LRR-NEL
    GAL4-VP16-GR
    

    Use

    Synthetic Biology

    Tags

    HA, Myc, and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    6xUAS-35Smini and pUBQ10

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-N2-Frankenbody-HA

    Plasmid

    #175292

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-HA frankenbody fused with mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQ173

    Plasmid

    #99907

    Purpose

    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Insert

    pco-dCas9-VP64-T2A-MS2-VP64
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SF-U7snRNA-CypA-E4

    Plasmid

    #190695

    Purpose

    Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

    Depositor

    Pim Pijnappel

    Article

    van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

    Insert

    U7 snRNA antisense CypA Exon 4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U7 promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 6691 Bicistronic_ires_puro

    Plasmid

    #64335

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMOD_B2103

    Plasmid

    #91061

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pMOD_B2303

    Plasmid

    #91068

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQ-dSpRY-Act3.0

    Plasmid

    #158416

    Purpose

    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQ265E2

    Plasmid

    #164719

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRIP CMV VHHG4

    Plasmid

    #182236

    Purpose

    Expresses GFP nanobody for stable expression in mammalian cells

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ141-ZmUbi-RZ-As

    Plasmid

    #86196

    Purpose

    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1 gRNA cloning site for ribozyme cleavage
    

    Use

    CRISPR

    Tags

    Hammerhead ribozyme and HDV ribozyme

    Expression

    Plant

    Promoter

    Maize ubiquitin 1

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

    Plasmid

    #160611

    Purpose

    Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:LbCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pEND-400_Cacnes_CAMP2

    Plasmid

    #225606

    Purpose

    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMOD_B2101

    Plasmid

    #91060

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYPQ-dpcoCas9-Act3.0

    Plasmid

    #158408

    Purpose

    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMOD_B2112

    Plasmid

    #91064

    Purpose

    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    PvUbi1

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMOD_B2203

    Plasmid

    #91066

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUTKan-3xHA-GFP-TOM5

    Plasmid

    #130674

    Purpose

    Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

    Depositor

    Andreas Weber

    Article

    Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

    Insert

    Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
    

    Tags

    3xHA and sGFP

    Expression

    Plant

    Promoter

    Ubiquitin10

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS

    Plasmid

    #123189

    Purpose

    binary plant vector for transient fLUC (with intron) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::fLUC-I::tNOS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYPQ-SpRY-Act3.0

    Plasmid

    #178957

    Purpose

    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge