We narrowed to 4,850 results for: U6...

1. pDest_U6_A2_CG2_T2_gRNA

   Plasmid

   #199388

   Purpose

   pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   nrg1 type II sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest_U6_A2_CG2_T3_gRNA

   Plasmid

   #199389

   Purpose

   pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   nrg1 type III sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHACK-QF2-AgU6-mCherry

   Plasmid

   #184530

   Purpose

   HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

   Depositor

   Christopher Potter

   Article

   Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

   Insert

   QF2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Anopheles U6 promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest_U6_A2_CG2_typeEGF_gRNA

   Plasmid

   #199386

   Purpose

   pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   nrg1 EGF sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HEK3_pegF_18bp

   Plasmid

   #174852

   Purpose

   Express forward pegRNA mediating 991-bp deletion and 18-bp insertion

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   HEK3_pegF_18bp insertion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. HEK3_pegR_18bp

   Plasmid

   #174853

   Purpose

   Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   HEK3_pegR_18bp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HEK3_pegF_44bp

   Plasmid

   #174854

   Purpose

   Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   HEK3_pegF_44bp insertion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HEK3_pegR_44bp

   Plasmid

   #174855

   Purpose

   Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   HEK3_pegR_44bp insertion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiSAMPHv2

   Plasmid

   #167934

   Purpose

   All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6.3>Control.gRNA.f+e

    Plasmid

    #99140

    Purpose

    Control gRNA under the regulation of an optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    Control gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Chicken U6.3

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-G-Creon shRNA[Control]

    Plasmid

    #181824

    Purpose

    This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LsgRNA-MS2

    Plasmid

    #235597

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-sgRNA1-2XMS2

    Plasmid

    #75389

    Purpose

    sgRNA1-2XMS2

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6.3>gRNA.f+e

    Plasmid

    #99139

    Purpose

    Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    chicken U6.3

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha and mU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide-TagBFP-2A-BSD

    Plasmid

    #167929

    Purpose

    Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDAS12069_U6-pegRNA-mCherry

    Plasmid

    #177180

    Purpose

    pegRNA cloning plasmid with mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge