We narrowed to 9,955 results for: EPO

1. pLenti-FNLSHiFiNG-P2A-Puro

   Plasmid

   #136903

   Purpose

   Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFiNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

   Plasmid

   #202571

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKanCMV-CX4.3-7aa-mRuby3

   Plasmid

   #74254

   Purpose

   Mammalian expression of C-terminal fusion of mRuby3 to CX43

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   Connexin 43
   

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   CAG and CMV

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGREAT2

   Plasmid

   #170890

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKanCMV-mClover3-18aa-actin

   Plasmid

   #74259

   Purpose

   Mammalian expression of N-terminal fusion of mClover3 to actin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   Actin
   

   Tags

   mClover3

   Expression

   Mammalian

   Promoter

   CAG and CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKanCMV-mRuby3-18aa-actin

   Plasmid

   #74255

   Purpose

   Mammalian expression of N-terminal fusion of mRuby3 to actin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   actin
   

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   CAG and CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDAS12342_PEAR-GFP_2in1

   Plasmid

   #177184

   Purpose

   PEAR-GFP plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKanCMV-mClover3-18aa-Tubulin

    Plasmid

    #74253

    Purpose

    Mammalian expression of N-terminal fusion of mClover3 to Tubulin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Tubulin
    

    Tags

    mClover3

    Expression

    Mammalian

    Promoter

    CAG and CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-CD81-(TEV)-tTA

    Plasmid

    #162598

    Purpose

    human CD81-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. A3Bctd-max

    Plasmid

    #198888

    Purpose

    Express A3Bctd-max BE4max-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Tags

    6x His and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRR-GR-5Z

    Plasmid

    #23060

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    GR (NR3C1 Human)
    

    Expression

    Yeast

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pIC306

    Plasmid

    #52257

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska3 (SKA3 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONOR-tagBFP-PSM-EGFP

    Plasmid

    #100603

    Purpose

    Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    EGFP-T2A-Puro and tagBFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGREAT26

    Plasmid

    #170914

    Purpose

    GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pEstradiol_Inducible

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

    Plasmid

    #200014

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. P447-FBP_19

    Plasmid

    #139710

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJSK418

    Plasmid

    #205131

    Purpose

    Expression of GFP-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    EGFP

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDONOR-tagBFP-PSM-dTOMATO

    Plasmid

    #100604

    Purpose

    Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    dTOMATO-T2A-Puro and tagBFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3-Basic-hCYP26A1P-E4-mCherry

    Plasmid

    #135478

    Purpose

    Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    mCherry Open reading Frame
    

    Use

    Unspecified

    Promoter

    Short from promoter of human CYP26A1 (312 bp)

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-InPAkt

    Plasmid

    #181914

    Purpose

    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    InPAkt
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRE-UBC-mCD59-T2A-GFP

    Plasmid

    #205451

    Purpose

    lentiviral plasmid for expression of mouse CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD59 (Cd59a Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. A3Bi-Cas9n-UGI

    Plasmid

    #119135

    Purpose

    Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3B L1 intron-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    L1 intron insertion

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIC292

    Plasmid

    #51405

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska2 IMAGE 4685972 (SKA2 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-Sox17

    Plasmid

    #60669

    Purpose

    PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Sox17 (Sox17 Mouse)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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