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Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTMP
Plasmid#129560Purposecytoplasmic markerDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-EcSsra
Plasmid#131217PurposeExpresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-EcSsra
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-pep
Plasmid#131215PurposeExpresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-pep
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
UseTagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL800
Plasmid#119306Purposeprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290SDepositorInsertprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
UseTagsnoneExpressionBacterialMutationamino acids 1 through 171 deleted, and mutatioms …PromoterlacAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
UseTagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-MCS-Avi
Plasmid#110205PurposeRecipient vector for expression of C-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choiceDepositorInsertENH_BsmB1-MCS-Avi
UseTagsAvi_tagExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-Avi-MCS-2A-Citrine
Plasmid#110204PurposeRecipient vector for expression of N-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice.DepositorInsertENH_BsmB1-Avi-MCS-2A-Citrine
UseTagsAvi_tag-2a-YFPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
UseTagsExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1 (220aa)
Plasmid#45287DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-151)
Plasmid#37308DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFPExpressionMammalianMutationcontains aa 1-151PromoterCMVAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
HIF-TRE-mStrawberry reporter
Plasmid#158681PurposeThis plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p35S:Citrine-TurboID-3xHA
Plasmid#199243PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID
UseTags3xHA and TurboID-3xHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
YAP/TAZ-TRE-mStrawberry reporter
Plasmid#158682PurposeThis plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p53-TRE-mStrawberry reporter
Plasmid#158684PurposeThis plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBQ:Citrine-TurboID-3xMyc
Plasmid#199244PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID-3xMyc
UseTagsTurboID-3xMycExpressionPlantMutationPromoterArabidopsis UBQ10 promoter with intronAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW003-Pc-mNeonGreen
Plasmid#205018PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Mitbow
Plasmid#158994PurposeMulticolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertMitbow
UseTagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC5
Plasmid#231654PurposeExpresses human ZDHHC5 proteinDepositorInsertZDHHC5 (ZDHHC5 Human)
UseTags3×Flag TagExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mNeonGreen
Plasmid#205016PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL/IFNB1-d2eGFP-3’UTR
Plasmid#180232PurposeReporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1DepositorInsertd2eGFP
UseLentiviralTagsExpressionMutationPromoter1 kb upstream of IFNB1 CDSAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xla.Tubb2:hArr-TEV;he1.1:eYFP
Plasmid#218859PurposeXla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
UseTagshuman Arrestin-Tobbaco Etch Virus protease fusion…ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1-IRES2-EGFP
Plasmid#170820Purposehuman GABAA receptors alpha1 subunit and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only