We narrowed to 5,033 results for: plasmid dna
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Plasmid#58534PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB ID1-iCreERT2
Plasmid#139280PurposeHuman ID1-iCreERT2 transgene piggyBac transposonDepositorInsertID1 (ID1 Human)
UseCre/LoxTagsExpressionMammalianMutationReplaced the ID1 protein coding sequence in the t…PromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJH027_SC101_pLAC_LbCas12a_Kan
Plasmid#183074PurposeWild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertLbCas12a
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH026_proD_Cas12c-multiplexing_CAM
Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterArabidopsis ubiquitin 10 (AtUbi10)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CIBN-CreC
Plasmid#26889PurposeExpresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.DepositorInsertCIBN-CreC
UseTagsExpressionMammalianMutationaa 1-170 of CIB1, aa 106-343 of CrePromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pP2A_NLS_sfGFP
Plasmid#63709PurposeExpressing P2A_NLS_sfGFP with homologous arm of Nanog.DepositorInsertNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
UseJust a donor dna with nanog homologous arm for kn…TagssfGFPExpressionMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP789_αGCN4-DNMT3A-CO
Plasmid#211783PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertaGCN4-DNMT3A
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP785_Traptavidin-DNMT3A-CO
Plasmid#211778PurposeAviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavidin-EZH2
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK193
Plasmid#71834PurposeCloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) regionDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UsePhage lambda cloning vector with genomic dna inse…TagsExpressionMutationadditional N-terminal methionine encoded by this …PromoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCasY1
Plasmid#87687Purposep15A plasmid expressing CasY1DepositorInsertCasY1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCX420
Plasmid#229211PurposeCytoplasmic-targeting Csm complex plasmidDepositorInsertCsm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Synaptotagmin-7 scFv [N275/14]
Plasmid#211608PurposeMammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from hybridoma N275/14.DepositorInsertSynaptotagmin-7 (Rattus norvegicus) recombinant mouse scFv. (Syt7 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Rem2 scFv [N324/2 scFv]
Plasmid#219491PurposeMammalian Expression Plasmid of anti-Rem2 (Rat) scFv. Derived from hybridoma N324/2.DepositorInsertAnti-Rem2 (Rattus norvegicus) recombinant mouse scFv. (Rem2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIP8b (exon 4) scFv [N212/3]
Plasmid#211607PurposeMammalian Expression Plasmid of TRIP8b (exon 4) scFv. Derived from hybridoma N212/3.DepositorInsertTRIP8b (exon 4) (Rattus norvegicus) recombinant mouse scFv. (Pex5l Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SynDIG1/Tmem90b scFv [L42/17]
Plasmid#211602PurposeMammalian Expression Plasmid of SynDIG1/Tmem90b scFv. Derived from hybridoma L42/17.DepositorInsertSynDIG1/Tmem90b (Rattus norvegicus) recombinant mouse scFv. (Syndig1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
QKI-6 scFv [N182/17]
Plasmid#211606PurposeMammalian Expression Plasmid of QKI-6 scFv. Derived from hybridoma N182/17.DepositorInsertQKI-6 (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-strong
Plasmid#108313PurposeGFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterpFAB4026Available sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-pBAD
Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-weak
Plasmid#108314PurposeGFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterpFAB4282Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
UseTagsExpressionBacterialMutationPromoterpFAB4005Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p006-Banana-Late
Plasmid#112251PurposeATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.DepositorInsertATF1 under osmY promoter
UseTagsExpressionBacterialMutationPromoterosmYAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH234-Tier1-PmPGK1-tTA
Plasmid#169574PurposeTier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-weak
Plasmid#108316PurposeSynthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.DepositorInsertRFP under controlf of weak synthetic constitutive promoter pFAB4024
UseTagsExpressionBacterialMutationPromoterpFAB4024Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pan-QKI scFv [N147/6]
Plasmid#211605PurposeMammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.DepositorInsertPan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-REEP1 scFv [N345/51 scFv]
Plasmid#219493PurposeMammalian Expression Plasmid of anti-REEP1 (Mouse) scFv. Derived from hybridoma N345/51.DepositorInsertAnti-REEP1 (Mus musculus) recombinant mouse scFv. (Reep1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGFAP-TetOn3G
Plasmid#133728PurposePlasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.DepositorInsertTetOn3G
UseAAVTagsExpressionMammalianMutationPromoterpGFAPAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAMKIIa-TetOn3G
Plasmid#133727PurposePlasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.DepositorInsertTetOn3G
UseAAVTagsExpressionMammalianMutationPromoterpCamk2aAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-hDAGLb-V5
Plasmid#87675PurposeExpresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminalDepositorInsertHuman Diacylglycerol Lipase Beta (DAGLB Human)
UseTagsHA and V5 tagExpressionMammalianMutationHemagglutinin (HA) peptide sequence (YPYDVPDYA) f…PromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRM56 tet-AR(1-707)C617Y
Plasmid#183504PurposeLentiviral vector expressing a doxycycline-inducible truncated DNA-binding mutant androgen receptor (AR mutant C617Y)DepositorInsertAndrogen receptor (AR Human)
UseLentiviralTagsExpressionMammalianMutationTruncated mutant AR; spans AR 1-707 aa, and conta…PromoterCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only