We narrowed to 5,443 results for: 129

1. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

   Plasmid

   #129532

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIA4
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. rpoDs366C

   Plasmid

   #129688

   Purpose

   Expression of Sigma 70 S366C

   Depositor

   Jeff Gelles

   Article

   Mumm et al bioRxiv 2020

   Insert

   rpoD (rpoD E. coli)
   

   Use

   Tags

   His6/thrombin

   Expression

   Bacterial

   Mutation

   C132S C291S C295S S366C

   Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBN449

   Plasmid

   #129555

   Purpose

   GAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegans

   Depositor

   Peter Askjaer

   Article

   Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

   Insert

   15xUAS-∆pes-10-Flp-SL2
   

   Use

   Tags

   mNeonGreen

   Expression

   Worm

   Mutation

   Promoter

   UAS-∆pes-10

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

   Plasmid

   #129667

   Purpose

   A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAN201

   Plasmid

   #129204

   Purpose

   yTRAP sensor of [RNQ+], detects aggregation of Rnq1

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   RNQ1 (RNQ1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV 3xFLAG-ATG3 C264A

   Plasmid

   #129297

   Purpose

   Expresses 3xFLAG-ATG3 C264A in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Cysteine 264 to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ290

   Plasmid

   #129670

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   AacCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1Donor_WT_ADRB2_LucReporter

   Plasmid

   #129788

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-PLEKHG4

   Plasmid

   #129618

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   PLEKHG4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LV-Camk2-Ace-8aa-mScarlet

    Plasmid

    #129702

    Purpose

    Expresses Ace-8aa-mScarlet in mammalian cells

    Depositor

    Yiyang Gong

    Article

    Beck et al Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.

    Insert

    Ace-8aa-mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Camk2

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Ace2_D92N_E199V-mNeon-ST

    Plasmid

    #129261

    Purpose

    Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mNeon-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAN147

    Plasmid

    #129202

    Purpose

    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129626

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLIPglu-2uDelta13

    Plasmid

    #12990

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F D152A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    D152A mutation in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid

    #129538

    Purpose

    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

    Depositor

    Arneet Saltzman

    Article

    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox; Mos1-mediated single copy insertion (mos…

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ292

    Plasmid

    #129672

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    AaCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPR v2 mBAK

    Plasmid

    #129578

    Purpose

    CRISPR deletion of murine BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAK (Bak1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-PB-PGK-tetR-KRAB-2A-Puro

    Plasmid

    #129536

    Purpose

    PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    PB-PGK-tetR-KRAB-2A-Puro
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid

    #129394

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV and Cre/Lox

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pB-RTP2-Integration

    Plasmid

    #129459

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP2 (RTP2 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-Ace2_D92N_E199V-mRuby3-ST

    Plasmid

    #129262

    Purpose

    Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mRuby3-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLBS.CAG/NLS-mScarlet

    Plasmid

    #129336

    Purpose

    nucleus marker, lentivirus

    Depositor

    Yasushi Okada

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

24. pGEX-4T1 NLS-GFP

    Plasmid

    #129441

    Purpose

    bacterial expression of GFP

    Depositor

    Oskar Laur

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMM0641

    Plasmid

    #129006

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T-1-3xMyc

    Plasmid

    #129569

    Purpose

    Empty backbone for Myc Tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFLIPglu-600uDelta13

    Plasmid

    #12991

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pN1-OPA1(123Nt)-Halo

    Plasmid

    #129345

    Purpose

    Halo Tag, Mitochondrial intermembrane space localization

    Depositor

    Yasushi Okada

    Insert

    Halo (Promega)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-PLEKHG2

    Plasmid

    #129617

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    PLEKHG2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge