-
Plasmid#172308PurposeIn vitro transcription of NGN2 (Ser phosphosites substituted by Ala)DepositorInsertNGN2
UseOtherTagsExpressionMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A an…PromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-Spastin(87-616)DeltaExon4
Plasmid#128794PurposeBacteiral expression of Spastin M87 varient without exon 4DepositorInsertSpastin (SPAST Human)
Use`TagsGSTExpressionBacterialMutationresidues 87-616 without exon 4.PromoterT7Available sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEX-CFP-STIM1-deltaK
Plasmid#18860DepositorInsertSTIM1 (STIM1 Human)
UseTagsCFPExpressionMammalianMutationStop codon at amino acid position 671 of STIM1. C…PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN, F124D
Plasmid#180648PurposeLentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, F124D mutation, has siRNA resistan…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-BclXL-Blasticidin
Plasmid#120718PurposeExpresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertBl2-like protein 1, isoform Bcl-X(L)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutation33 c-terminal amino acidsPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 K11,12R GFP
Plasmid#35412DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationK11,12RPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pecALPS gag-gfp Blasti
Plasmid#115805PurposeExpress gag-gfp fusion driven by EIF1a promoterDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterEIF1aAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptcALPS gag-gfp Blasti
Plasmid#115806PurposeExpress gag-gfp fusion driven by TK promoterDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterTKAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Collybistin [L120/12R]
Plasmid#177575PurposeMammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/12.DepositorInsertanti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn Arrestin-TEVp-V5
Plasmid#173118PurposeExpresses arrestin receiver construct in neuronsDepositorInsertArrestin-TEVp-V5
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL ∆CC
Plasmid#80268Purposemammalian expression plasmid for MycLAP-STIL coiled-coil deletionDepositorInsertSTIL (STIL Human)
UseTagsMyc-GFPExpressionMammalianMutationmissing conserved coiled-coil (amino acids 721-74…PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-30-picky_sticky
Plasmid#138984PurposeIVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Collybistin [L120/30R]
Plasmid#177465PurposeMammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/30.DepositorInsertanti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-TCRb-FKBP-Blasticidin
Plasmid#120723PurposeExpresses ER-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertT cell receptor β chain
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX395 Roseburia intestinalis Cas9
Plasmid#68332PurposeRoseburia intestinalis Cas9DepositorInsertRiCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
KJ201: pMVP (R4-R3) osTir1 open
Plasmid#121743PurposepMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1 (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc epitope tagExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-mSTING (1-339)
Plasmid#229740PurposeExpresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.DepositorInsertSTING (Sting1 Mouse)
UseTagsNoneExpressionMammalianMutationContains amino acids 1-339PromoterEF1AAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_AAVS1-OsTIR1-F74G-SNAP
Plasmid#217665PurposeRepair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locusDepositorInsertOsTir1 F74G-SNAP-Blast-AAVS1
UseTagsSNAPExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Blasticidin XLone-eGFP
Plasmid#159754PurposeAAVS1 donor plasmid for targeted inducible eGFP expression in human cellsDepositorInsertall-in-one tet-on system
UseCRISPR and TALEN; Donor plasmid for targeted knoc…TagsExpressionMammalianMutationPromoterTRE3GS inducible promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)
Plasmid#184716PurposeOptoSTIM1(CRY2clust)DepositorInsertOptoSTIM1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR
Plasmid#200639PurposeLow STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN, L177D
Plasmid#180649PurposeLentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, L177D mutation, has siRNA resistan…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A-ZebrafishSTING
Plasmid#196633PurposeExpress Zebrasfish STINGDepositorInsertZebrafishSTING (sting1 Zebrafish)
UseLentiviralTagsmtagBFPExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6MYC osTIR1
Plasmid#193320PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6MYCExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6FLAG osTIR1
Plasmid#182515PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM254 - sgRNA mosTI neoR
Plasmid#159831PurposeSingle copy or array insertion by MosTI using split NeoR selectionDepositorInsertsgRNA mosTI neoR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.PstI-p15A-aadA
Plasmid#149340Purposeexpresses M.PstI DNA methyltransferase in E. coliDepositorInsertM.,PstI
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY1656 STITCHR Gluc AAVS1 payload
Plasmid#234827PurposeGluc exon 2 with 50bp AAVS1 homology armsDepositorInsertGluc exon 2
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDY1440 STITCHR GFP NOLC1 payload
Plasmid#234829PurposeGFP cargo with 50bp NOLC homology armsDepositorInsertcopGFP
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only