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pCMV4-ApoE4

Plasmid

#87087

Purpose

Expresses ApoE4 in Mammalian cells

Depositor

Bradley Hyman

Publication

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Apolipoprotein E4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti-CMV-MCS-GFP-SV-puro

Plasmid

#73582

Purpose

Third generation lentiviral vector expressing GFP and puromycin

Depositor

Paul Odgren

Publication

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Type

Empty backbone

Use

Lentiviral

Tags

GFP tag

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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Tubp-Gal80 in pCaSpeR4

Plasmid

#17748

Depositor

Liqun Luo

Publication

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL80 (GAL80 Budding Yeast)

Tags

SV40 polyA and Tubulin 1alpha promoter

Expression

Insect

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Publication

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Publication

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCMV4-ApoE3

Plasmid

#87086

Purpose

Expresses ApoE3 in Mammalian cells

Depositor

Bradley Hyman

Publication

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Apolipoprotein E3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLV-EF1a-IRES-Neo

Plasmid

#85139

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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FLAG-Pol-II 31-52

Plasmid

#35179

Depositor

Benjamin Blencowe

Publication

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of C-terminal domain repeats 1-30

Promoter

CMV

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
FUW-SOKM

Plasmid

#20325

Purpose

Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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EP-Pol vector

Plasmid

#131686

Purpose

A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Publication

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Mutation

F421Y, D827A

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Publication

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Tags

14Histidine-TEV

Expression

Bacterial

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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3XFlag DVL2 (WT)

Plasmid

#24802

Depositor

Jeff Wrana

Publication

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

16 Articles

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pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pIRESpuro-Flag-Pol b

Plasmid

#23257

Depositor

Robert Sobol

Publication

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DNA polymerase beta (POLB Human)

Tags

Flag

Expression

Mammalian

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Publication

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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Renilla luciferase-Pol III

Plasmid

#37380

Depositor

Norbert Perrimon

Publication

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PolIII-Renilla control reporter (RpIII128 Fly)

Use

Luciferase

Expression

Mammalian

Promoter

RNA PolIII 128 subunit (RpIII128)

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Publication

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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