We narrowed to 2,405 results for: dcas9
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Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG dCas9-KRAB-2A-EGFP
Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9_KRAB_2A_EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianPromoterCAGAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1
Plasmid#138419PurposeCAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1DepositorInsertdCas9
UseLentiviralTagsBioTAP-tagPromoterEF1aAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_118
Plasmid#113667PurposeLentiviral vector expressing dCas9DepositorInsertdCas9
UseCRISPR and LentiviralMutationcatalytically inactiveAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-dCas9
Plasmid#75381PurposedCas9DepositorInsertSp dCas9
UseLentiviralTagsnoExpressionMammalianPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorPublicationInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840AAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC84-pCR8-dCas9
Plasmid#48218PurposedCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A)
UseCRISPR and Gateway CloningTagsHA TagMutationD10A;H840AAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ezh2[FL]-dCas9
Plasmid#100086PurposeFull-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsert14056 (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[FL]-dCas9-NLSExpressionMammalianPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DNMT3A-dCas9
Plasmid#100090PurposeDNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3A (DNMT3A Human)
UseCRISPRTags3XFLag-NLS-DNMT3A-dCas9-NLSExpressionMammalianMutationincludes aa 602-912PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti SYN-dCas9-KRAB-MeCP2
Plasmid#155365PurposeExpresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoterDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTags3x FLAG and 7x HisExpressionMammalianPromoterSYNAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pnos-Fok1:dCas9-nos
Plasmid#62210PurposeExpresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
ExpressionInsectPromoternanosAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1
Plasmid#138417PurposeCAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1DepositorInsertsBirA
dCas9
UseLentiviralTagsFLAG, Avi-tag and V5, HisPromoterEF1aAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Neisseria meningiditis, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LLP457 pGK-dCas9-Suntag-BFP
Plasmid#100957PurposeBPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tagDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHA and BFPExpressionMammalianMutationD10A, H840A for dCas9Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by