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Plasmid#111518PurposeElectrophysiology in HEK cellDepositorInsertcalmodulin [Xenopus laevis] (calm2 Frog)
UseTagsExpressionMammalianMutationD20A/D56AD93A/D129APromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSFI-R-L301S-IRES-GFP
Plasmid#133744Purposeretroviral expression of CSF1-R mutantDepositorInsertCSF1-R (CSF1R Human)
UseRetroviralTagsExpressionMammalianMutationL301SPromoterMSCVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationdeleted bromodomainPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2
Plasmid#113890PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pGK-nAChRb2(E61C)-IRES-GFP
Plasmid#164777Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseLentiviralTagsExpressionMammalianMutationE61CPromoterpGKAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast 3xFlag-SPNS1(E164K)
Plasmid#198569PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
Plasmid#128387PurposeExpresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsCLuc394 and NLuc398ExpressionMammalianMutationPromoterEFS and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
Plasmid#75122PurposeRetroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …ExpressionMutationPromotereno2 from zebrafishAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST Halo-flag-CAD
Plasmid#188117PurposeExpresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tag and Halo tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2
Plasmid#113889PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
Plasmid#18810DepositorInsertmGluR6 (200bp crit)+SV40p-tdTomato-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-NGN2-IRES-GFP-T2A-Sox11
Plasmid#90213PurposeTo convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.DepositorUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-CD4-TLR4 cyto
Plasmid#133064PurposeRetroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4DepositorInsertCD4-TLR4cyto (Tlr4 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-TLR4 delta tail
Plasmid#133063PurposeRetroviral expression of mouse TLR4 with out its cytosolic tailDepositorInsertTLR4 delta tail (Tlr4 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K107/113R-3xFlag_IRES-GFP
Plasmid#198392PurposeRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site double mutant (K107/113R)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65
Plasmid#32569DepositorInsertsEGFP
Gal4p65
UseLentiviralTagsExpressionMutationPromoter2TPHAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L
Plasmid#122991PurposeExpresses Flag-tagged hRPGRIP1L in mammalian cellsDepositorInsertRPGRIP1L (RPGRIP1L Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only