We narrowed to 3,709 results for: Braf;

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pT2-cryR;yrkCE1-P1Egfp

Plasmid

#90156

Purpose

contains yrk enhancer sequence, and yrk specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkE1P1Egfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;snx5CE1-P1Egfp

Plasmid

#90152

Purpose

contains snx5 enhancer sequence, and snx5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5E1P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;yrkCE1-basEgfp

Plasmid

#90142

Purpose

contains yrk enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkE1basEgfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700

Plasmid

#64046

Purpose

Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6, CAGGS

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2-kdrl:mCherry-caax

Plasmid

#194287

Purpose

Expression plasmid for zebrafish or Danionella cerebrum

Depositor

Pui-Ying Lam

Article

Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

Insert

kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)

Use

Zebrafish or danionella cerebrum expression

Tags

mCherryCAAX from the Tol2kit (Kwan et al., 2007)

Promoter

kdrl(flk1)

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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sox10:PDGFRA wild-type

Plasmid

#98696

Purpose

I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDestTol2CR-QUAS:NTR-mKate2

Plasmid

#241914

Purpose

Zebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUAS enhancer driving nitroreductase-mKate2

Use

Zebrafish tol2 transgenesis

Tags

nitroreductase fused with mKate2

Promoter

QUAS

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

Plasmid

#218860

Purpose

10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCG-ICAM(1235)-nrxn1b

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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elavl3:hArr-TEV;he1.1:YFP

Plasmid

#218855

Purpose

D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hArr

Tags

human Arrestin-Tobbaco Etch Virus protease fusion…

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDrVSP-IRES2-EGFP

Plasmid

#80333

Purpose

Expresses Danio rerio VSP with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Article

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-ctrl-guides

Plasmid

#168240

Purpose

"neutrophil specific GFP with ubiquitous ctrl sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

control sgRNAs

Use

CRISPR

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

Plasmid

#191500

Purpose

GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol2 transgenes…

Promoter

myo6b

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hArr-TEV;he1.1:eYFP

Plasmid

#218859

Purpose

Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hArr

Tags

human Arrestin-Tobbaco Etch Virus protease fusion…

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Tags

Citrine

Expression

Mammalian

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

Plasmid

#238374

Purpose

Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting cmlc2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_erbb2_cmlc2-nmKate

Plasmid

#238409

Purpose

Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RR

Plasmid

#37276

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

Depositor

David Grunwald

Article

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pHSG1C3

Plasmid

#164423

Purpose

single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Tags

RFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-WHaloCaMP1a

Plasmid

#205312

Purpose

Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

Zebrafish expression

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p13xQUAS-MCS, α-cry:mCherry

Plasmid

#180481

Purpose

Inducible gene expression vector p13xQUAS-MCS, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

Multiple cloning site

Use

Synthetic Biology

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

Plasmid

#218206

Purpose

Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

+15.2prox1a

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-ABEmax

Plasmid

#164415

Purpose

EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

ABEmax

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mCerulean3

Plasmid

#127592

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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