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We narrowed to 12,235 results for: Hal

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Showing: 3601 - 3640 of 12235 results

  13. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid
    #121840
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121840-plasmid-map-sequence-id-237394

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  14. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121803
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121803-plasmid-map-sequence-id-237312

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  15. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121807
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121807-plasmid-map-sequence-id-237316

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  16. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid
    #121811
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121811-plasmid-map-sequence-id-237356

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  17. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121815
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121815-plasmid-map-sequence-id-237362

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  18. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121820
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121820-plasmid-map-sequence-id-237373

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  20. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid
    #121748
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    OLLAS epitope tag-IRES2-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121748-plasmid-map-sequence-id-237186

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  31. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

    Plasmid
    #214950
    Purpose
    Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.
    Depositor
    Dan Mulvihill
    Article
    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1
    Inserts
    VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
    VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
    Tags
    6xHis, Amino half of venus BIFC fragment, Carboxy…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    214950-plasmid-map-sequence-id-439535

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  38. pDONR223_HyPer7RgDAAO

    Plasmid
    #217746
    Purpose
    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217746-plasmid-map-sequence-id-442633

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Showing: 3601 - 3640 of 12235 results