We narrowed to 171,035 results for: addgene
-
-
mc2 mCherry EF1a
Plasmid#206225PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PFKL_pCSdest
Plasmid#53901DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
miR-vector-RFP pCDNA
Plasmid#97143Purposefor expression of miRNA and TagRFPDepositorTypeEmpty backboneTagsTagRFPExpressionMammalianAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MRAP_pCSdest
Plasmid#53833DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
ExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP1_2-mCherry
Plasmid#159526PurposeExpresses mCherry from LAP1_2 promoterDepositorInsertmCherry
UseAAVExpressionMammalianPromoterLAP1_2Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GalT-moxDendra2
Plasmid#89789Purposemammalian expression of GC localized moxDendra2DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long UTR
Plasmid#21649DepositorAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT7-AsCas12a_crRNA-site4 (MSP3511)
Plasmid#160139PurposeT7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4DepositorInsertAsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
UseIn vitro transcription of sgrna from t7 promoterPromoterT7Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
Tags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-PSPC1
Plasmid#46321DepositorAvailable SinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SLC5A3_pCSdest
Plasmid#53836DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT 3xFLAG Luciferase
Plasmid#98391PurposeGateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tagDepositorInsertLuciferase
UseEntry vector for gateway cloningTags3xFLAGPromoterTRE tight and TRE tight promoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG36-ChAT
Plasmid#99069PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)DepositorInsertChAT (choline acetyltransferase) in situ probe
UseT/a cloning vector for dsrna generation and the g…Available SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast
Plasmid#206217PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV CDHblast Rev-EGFP-V5
Plasmid#175164PurposeLentiviral expression of Rev-EGFP-V5DepositorInsertrev
UseLentiviralTagsV5ExpressionMammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_GAL4UAS-Luciferase reporter
Plasmid#64159PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA3 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck
Plasmid#58524PurposeExpresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cellsDepositorInsertsdChR(C138S, E154A)-TS-GCaMP6f-ER
UseLentiviralTagsGCaMP6fExpressionMammalianMutationchanged Cysteine 138 to Serine; changed Glutamate…Promotera-CamKIIAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-3HA
Plasmid#39351DepositorUseYeast genomic targetingTags3xHA and kanMX6 module - reverse orientationAvailable SinceJan. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab33bb in pCSDEST2
Plasmid#140882PurposeExpression of zebrafish GFP-tagged Rab33bb in mammalian cells.DepositorInsertRab33bb
TagsEGFPExpressionMammalianAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPERK
Plasmid#24826DepositorAvailable SinceJune 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_1
Plasmid#73525PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRExpressionMammalianPromoterhU6Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
YSEHPTFTSQY_epitope
Plasmid#165016PurposeExpression of CMV UL83 (363-373) fused to IL-2 signal sequenceDepositorInsertCMV UL83 (363-373)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianPromoterCMVAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (Puro) p65 shRNA2
Plasmid#22508DepositorAvailable SinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_RAB9A
Plasmid#111693PurposeMAC-tagged gene expressionDepositorInsertRAB9A (RAB9A Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM2937
Plasmid#84112Purposetarget miRNA2937 for destruction in Arabidopsis thalianaDepositorInsertSTTM2937
ExpressionPlantPromoter2x35SAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pLKO.1 EB1 shRNA #3
Plasmid#37927DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
px330-USP13
Plasmid#61812Purposeto make USP13 Knockout cell lineDepositorAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MRPS6_pCSdest
Plasmid#53824DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFGC5941-PacI-ath-STTM159
Plasmid#84084Purposetarget miRNA159 for destruction in Arabidopsis thalianaDepositorInsertSTTM159
ExpressionPlantPromoter2x35SAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG_GI4
Plasmid#111595PurposeGI Library VectorDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseVector for homologous recombinationPromoterTEF promoterAvailable SinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseVector for homologous recombinationPromoterTEF promoterAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir144 EF1Alpha-puro-T2A-BFP
Plasmid#164791PurposeExpress miR-144 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralExpressionMammalianPromoterEF1Alpha and U6Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG_GI2
Plasmid#111593PurposemU6-sgRNA GI Library vectorDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only
