We narrowed to 4,132 results for: pCMV mammalian

1. Bxb1-surrogate-reporter

   Plasmid

   #65624

   Purpose

   Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attP
   

   Use

   Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Aquamarine-LC3B-TdLanYFP

   Plasmid

   #228560

   Purpose

   Encodes the LC3B biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter

   Depositor

   Giulia Bertolin

   Article

   Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Tags

   Aquamarine and TdLanYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pR2Q358y

   Plasmid

   #101028

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   Glutamate receptor subunit (Gria2 Rat)
   

   Tags

   fluorescent protein

   Expression

   Mammalian

   Mutation

   tagged with fluorescent protein

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pR2Q347y

   Plasmid

   #112680

   Purpose

   functional, fluorescent, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   Glutamate receptor subunit (Gria2 Rat)
   

   Tags

   fluorescent protein

   Expression

   Mammalian

   Mutation

   tagged with fluorescent protein

   Promoter

   cmV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. cmv hTid Short

   Plasmid

   #13709

   Depositor

   Karl Munger

   Article

   Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

   Insert

   hTid Short (DNAJA3 Human)
   

   Expression

   Mammalian

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. p3xFLAG-MKL1 D301-380

   Plasmid

   #19852

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 301 to 380

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

7. cmv hTid Long

   Plasmid

   #13707

   Depositor

   Karl Munger

   Article

   Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

   Insert

   hTid Long (DNAJA3 Human)
   

   Expression

   Mammalian

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pPKm-112

   Plasmid

   #90494

   Purpose

   pcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   MTAD-PIF3
   

   Expression

   Mammalian

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pR2Q306c

   Plasmid

   #101027

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   Glutamate receptor subunit (Gria2 Rat)
   

   Tags

   fluorescent protein

   Expression

   Mammalian

   Mutation

   tagged with fluorescent protein

   Promoter

   CMV

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR2Q144c

    Plasmid

    #101023

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    CMV

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3xFLAG-MKL1 D381-506

    Plasmid

    #19853

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 381 to 506

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pR2Q105y

    Plasmid

    #101026

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Aquamarine-LC3B G120A-TdLanYFP

    Plasmid

    #228561

    Purpose

    Encodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    Aquamarine and TdLanYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Src(KD/YF)-SsrA(Y7I)-mCer3

    Plasmid

    #221000

    Purpose

    this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA, mCerulean3 (Src Mouse)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D388R/Y529F in Src, Y7I in SsrA

    Promoter

    pCMV

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pR2Q191y

    Plasmid

    #101029

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pR2Q207y

    Plasmid

    #101024

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pR2Q133c

    Plasmid

    #101025

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    CMV

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPKm-113

    Plasmid

    #90495

    Purpose

    pcDNA3 -pCMV - MTAD - PIF6, expresses the Phytochrome interacting factor 6 (PIF6) and MTAD, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    MTAD-PIF6
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Magoh198delG

    Plasmid

    #30481

    Depositor

    William Pavan, Debra Silver

    Article

    Silver et al Nat Neurosci. 2010 May . 13(5):551-8.

    Insert

    Magoh (Magoh Mouse)
    

    Expression

    Mammalian

    Mutation

    198delG (point nucleotide deletion)

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5-FRT-TO-NOD2-EGFP

    Plasmid

    #131207

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD2 (NOD2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. miniCGBE1-SpRY (pBM1571)

    Plasmid

    #170105

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

    Promoter

    CMV

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TALED_Left-mouse-ATP6-1397N.

    Plasmid

    #212951

    Purpose

    Expresses TALED_Left-mouse-ATP6-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TALED_Right-Cox3.1-1397C-AD.

    Plasmid

    #212953

    Purpose

    ExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Right-Cox3.1-1397C-AD
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TALED_Left-Cox3.1-1397N.

    Plasmid

    #212950

    Purpose

    Expresses TALED_Left-Cox3.1-1397N. in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Left-Cox3.1-1397N
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Venus1-WTSTAT3

    Plasmid

    #123164

    Purpose

    Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    none

    Promoter

    pCMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5-FRT-TO-NOD1-EGFP

    Plasmid

    #131206

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD1 (NOD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SPACE (pRZ1813)

    Plasmid

    #140242

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hyABE

    Plasmid

    #196096

    Purpose

    Expresses hyABE

    Depositor

    Xiahui Zhang

    Article

    Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

    Insert

    hyABE
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PEA1-Nuclease-Puro

    Plasmid

    #171992

    Purpose

    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

    Plasmid

    #111502

    Purpose

    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    ZF(VEGFA)-StaPL(AI)-YFP-VPR
    

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries V36M, T54A, and S122…

    Promoter

    CMV

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDmelOR

    Plasmid

    #126476

    Purpose

    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    DmelOrco (Orco Fly)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens; includes a rec…

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PEA1-Nuclease-GFP

    Plasmid

    #171994

    Purpose

    Delivers all prime editing nuclease components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pS6-TGFB2-H8

    Plasmid

    #128502

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (L21-S414) (TGFB2 Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SPACE-VRQR (pRZ5137)

    Plasmid

    #140245

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_VRQR
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, VRQR mutations in SpCas9, D10A in …

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TALED_Left-ND6-1397N

    Plasmid

    #212952

    Purpose

    Expresses TALED_Left-ND6-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Left-ND6-1397N
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TALED_Right-ND6-1397C-AD

    Plasmid

    #212956

    Purpose

    Expresses TALED_Right-ND6-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Right-ND6-1397C-AD
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CMV-AID'-seBE4max-IRES

    Plasmid

    #174697

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    AID'-seBE4max-IRES (AICDA Human)
    

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    UGI-E11K

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge