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Plasmid#68588PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only
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pMpGWB334
Plasmid#68662PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU324
Plasmid#167691PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-LAMP1-mGL
Plasmid#222394PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.DepositorInsertLAMP1 and mGreenlantern (LAMP1 Synthetic, Homo Sapiens, Human)
UseAAVTagsLAMP1 and mGreenLanternExpressionMammalianMutationPromoterCAG or CAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB104
Plasmid#68558PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB107
Plasmid#68561PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LRS-L-11
Plasmid#197720PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LRSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-Cre-GR-9
Plasmid#197723PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCre recombinase with St-LS1 intron fused to glucocorticoid receptor
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ZmBbm-P2A-ZmWus2-9
Plasmid#197750PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm-P2A-ZmWus2 bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB101
Plasmid#68555PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-mCh-empty
Plasmid#21907PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCsA
Plasmid#136067PurposeAcceptor plasmid for even level position 1, lacZ blue-white screeningDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D2.0
Plasmid#99906PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
L1_HyR-Ck1
Plasmid#136125PurposeL1 in position 1 (next to LB in L2 construct), for HygRDepositorInsertp5-35S:hptII_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9-D10A
Plasmid#61477Purposebased on pDe-CAS9, encodes Cas9 D10A nickase variantDepositorInsertCas9-D10A
UseCRISPRTagsExpressionPlantMutationcatalytic Asp10 changed to AlaPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:LUC
Plasmid#131860PurposeTransient expression of firefly luciferase from the Zea mays ubiquitin promotorDepositorInsertluciferase
UseTagsExpressionPlantMutationPromoterZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-WDV-Rep-RepA-9
Plasmid#197716PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV Rep/RepA proteins: native ORFs
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-BeYDV-LS-L-11
Plasmid#197717PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV LSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-BeYDV-LRS-L-11
Plasmid#197718PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV LRSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LS-L-11
Plasmid#197719PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB103
Plasmid#68557PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK019
Plasmid#105378PurposeSynthetic biologyDepositorInsertpromotor EXP7 (At1g12560; 750bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB102
Plasmid#68556PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB229
Plasmid#68620PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-RedFLuc-9
Plasmid#197738PurposePhytobrick (MoClo) Level 0 PartDepositorInsertRedF Luciferase (tomato optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRU206
Plasmid#167693PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU322
Plasmid#167683PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPS2
Plasmid#209972PurposeContains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmidsDepositorInsertRBS (Lp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB304
Plasmid#68632PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SPb
Plasmid#83205Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertTomato ACTIN promoter
UseTagsExpressionPlantMutationPromoterTomato ACTINAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ii-OVA.II
Plasmid#64597PurposeExpression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentationDepositorInsertOvalbumin
UseTagsInvariant chainExpressionMammalianMutationOnly amino acids 315-353 (class II epitopes)PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:GW
Plasmid#132358PurposeExpression of CDS from the Zea mays ubiquitin promotorDepositorTypeEmpty backboneUseTagsYFPExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105
Plasmid#68559PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionTagsExpressionMutationRice codon optimizedPromoter2x35SAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB208
Plasmid#68599PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SP
Plasmid#83204Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertSalI/PstI cloning sites
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PROM5_35Sx2
Plasmid#136112PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35Sx2 (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35S
Plasmid#136111PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35S (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk2_spacer
Plasmid#136072PurposeUse when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-UbC-mCherry Rab5
Plasmid#115239PurposeTo express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.DepositorInsertRab5a (RAB5A Human, Homo sapiens)
UseAAVTagsmCherryExpressionMammalianMutationPromoterHuman UbCAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB308
Plasmid#68636PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN119
Plasmid#176884PurposeEncodes STM and WUS2 for ectopic meristem induction in plantsDepositorInsertCmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits