We narrowed to 5,472 results for: chia

1. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SS9_RNA

   Plasmid

   #71656

   Purpose

   SS9-targeting gRNA for co-transformation with pSS9

   Depositor

   Ryan Gill

   Article

   Bassalo et al ACS Synth Biol. 2016 Apr 22.

   Insert

   SS9 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30del

   Plasmid

   #106902

   Purpose

   pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKIKOrbsARCm

   Plasmid

   #46765

   Purpose

   E.coli genomic integration rbsAR locus

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG022 (dsr1)

   Plasmid

   #157900

   Purpose

   Expresses the dsr1 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   dsr1 (SIR2-DUF4020)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW071

   Plasmid

   #140037

   Purpose

   PCC 11901 fadD locus KO with a kanamycin resistance cassette

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Kanamycin resistance gene
   

   Use

   Gene knockout in cyanobacteria

   Tags

   Expression

   Mutation

   Promoter

   KanR

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKIKOrbsARKm

   Plasmid

   #46768

   Purpose

   E.coli genomic integration rbsAR locus

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTR(hCc)

   Plasmid

   #61026

   Purpose

   Expresses horse holocytochrome c in E. coli

   Depositor

   Gary Pielak

   Article

   Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

   Insert

   horse cytochrome c (CYCS Equus*)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   *see comments

   Promoter

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHEA

   Plasmid

   #168297

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

   Insert

   Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
   

   Use

   Tags

   6xHis, E-tag, and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG016 (ApeA)

    Plasmid

    #157894

    Purpose

    Expresses the ApeA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    ApeA (putative HEPN RNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI) promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbA5c-MevT-MBI (JBEI-3085)

    Plasmid

    #87950

    Purpose

    the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    MevT-MBI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMUT2-pLac-mCherry

    Plasmid

    #192859

    Purpose

    pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    lldR operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJM.683

    Plasmid

    #108531

    Purpose

    BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    BetIAM + PBetI-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BetI: S115I

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28a-ClbB-NRPS-NHis

    Plasmid

    #49217

    Purpose

    NRPS module of ClbB containing CAT domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbB-NRPS
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa4-1080 of reference sequence NP_754362…

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDsEndoNA2

    Plasmid

    #174728

    Purpose

    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

    Insert

    DsRed-Monomer
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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