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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pSL0527 (pDonor)

Plasmid

#130634

Purpose

Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (Mini-Tn)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

6xHis and mCardinal

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1/nV5-DEST-beta catenin

Plasmid

#20140

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

T3I

Promoter

CMV

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Article

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRFHUE-eGFP

Plasmid

#89469

Purpose

GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

Depositor

Luis González-Candelas

Article

Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

Insert

eGFP

Use

Binary plasmid for atmt of filamentous fungi

Promoter

A. nidulans gpdA

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-GPI

Plasmid

#65294

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ1B

Plasmid

#65372

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ1B (BAZ1B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mLmx1a

Plasmid

#33013

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V-NC-MS2

Plasmid

#122944

Purpose

MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

NC-MCP (MS2 coat protein) fusion protein in Gag

Use

Lentiviral

Expression

Mammalian

Mutation

D64V

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-PV-NES-GFP

Plasmid

#17301

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-NES-GFP (Pvalb Rat)

Tags

Myc

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDAC627

Plasmid

#195240

Purpose

Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCLE NICD3

Plasmid

#26894

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD3 (NOTCH3 Human)

Use

Retroviral

Tags

Alkaline Phosphatase and IRES

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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