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pJC2743 - hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250274

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-MTFR1L(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-TOP3B (Y336F)-3xFLAG

Plasmid

#249682

Purpose

Expresses human TOP3B (Y336F; catalytically inactive mutant) with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Michael Kearse

Article

Warrick et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

human TOP3B (TOP3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y336F

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-TOP3B (R338W)-3xFLAG

Plasmid

#249683

Purpose

Expresses human TOP3B (R338W; "self-trapping" mutant) with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Michael Kearse

Article

Warrick et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

human TOP3B (TOP3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-TOP3B (WT)-3xFLAG

Plasmid

#249681

Purpose

Expresses human TOP3B (wildtype) with a C-terminal 3xFLAG tagged in mammalian cells

Depositor

Michael Kearse

Article

Warrick et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

human TOP3B (TOP3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-TOP2B

Plasmid

#244334

Purpose

5' AAVLINK plasmid for TOP2B expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

TOP2B (TOP2B Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AAV Syn-FLEX-coca-GlyR-IRES-mCherry

Plasmid

#242196

Purpose

Cre-dependent AAV vector for cocaine-gated chemogenetic anion channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-FLEX-coca-GlyR-IRES-mCherry

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/V122I

Plasmid

#238122

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, V122I

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/V30M

Plasmid

#238119

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, V30M

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244089

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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BCL2-NanoLuc Fusion Vector

Plasmid

#238867

Purpose

Express BCL2-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

BCL2 (BCL2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-JAK3 Vector

Plasmid

#238703

Purpose

Express NanoLuc-JAK3 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

JAK3 (JAK3 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET Assay Vector, NanoLuc-KRas(G12D)

Plasmid

#238581

Purpose

Express NanoLuc-KRas(G12D) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

KRAS (KRAS Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-FLEX(cre)-OCaMP-WPRE

Plasmid

#229853

Purpose

pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Aug. 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Sc rev7-pRS405/GAL

Plasmid

#241259

Purpose

Over-express Sc rev7 (Sc Pol zeta subunit) in yeast (integrated)

Depositor

Michael O'Donnell

Insert

rev7 (REV7 Budding Yeast)

Expression

Yeast

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-flx-fDIO-HA-Rpl10a-T2A-myc-hM3D(Gq)

Plasmid

#223654

Purpose

Expresses vTRAP and hM3D(Gq) in a FlpON-CreOFF dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

vTRAP-T2A-hM3D(Gq)

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE2iso4_Z

Plasmid

#148119

Purpose

Mammalian Expression of HshnRNPE2iso4

Depositor

Elisa Izaurralde

Insert

HshnRNPE2iso4 (PCBP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P9-ehaA_LacI

Plasmid

#186317

Purpose

Plasmid for inducible E coli expression of the P9 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P9-ehaA

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAF9D92

Plasmid

#185846

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>TUP1>PABF1

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP10-ehaA_LacI

Plasmid

#186318

Purpose

Plasmid for inducible E coli expression of the AP10 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP10-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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