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pLCS110

Plasmid

#209331

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mStayGold fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Gm-SceI-pheS

Plasmid

#111609

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLCS109

Plasmid

#209330

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-EGFP

Plasmid

#65300

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-HisRS-His

Plasmid

#124111

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Histidyl-tRNA ligase (hisS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-MetRS-His

Plasmid

#124115

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionine--tRNA ligase (metG Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-ProRS-His

Plasmid

#124117

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Prolyl-tRNA ligase (proS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-AspRS-His

Plasmid

#124106

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Aspartate-tRNA ligase (aspS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TyrRS-His

Plasmid

#124121

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tyrosyl-tRNA ligase (tyrS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-SerRS-His

Plasmid

#124118

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Seryl-tRNA ligase (serS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDAC627

Plasmid

#195240

Purpose

Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-His-ArgRS

Plasmid

#124104

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Argin+I4 ligase (argS Escherichia coli)

Tags

10x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LeuRS-His

Plasmid

#124113

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Leucyl-tRNA ligase (leuS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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