4,180 results

1. pTetON-EGFP

   Plasmid

   #89453

   Purpose

   Tet/Dox-inducible expression of EGFP in mammalian cells (control)

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-puro FLAG SREBP2

   Plasmid

   #32018

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG/MNase

   Plasmid

   #123461

   Purpose

   Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

   Depositor

   Steven Henikoff

   Article

   Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

   Insert

   Protein A-Protein G-Micrococcal Nuclease
   

   Tags

   6xHIS and HA

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti.PGK.LifeAct-Ruby.W

   Plasmid

   #51009

   Purpose

   Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   LifeAct-mRFPruby
   

   Use

   Lentiviral

   Tags

   LifeAct

   Expression

   Mammalian

   Promoter

   human PGK

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flamindo2

   Plasmid

   #73938

   Purpose

   Expresses biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   Flamindo2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. anti-FLAG M2 heavy chain

   Plasmid

   #175359

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 heavy chain
   

   Tags

   His8 tag and N-terminal secretion signal peptide …

   Expression

   Mammalian

   Mutation

   L51I (see paper)

   Promoter

   CMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynap.iGABASnFR

   Plasmid

   #112159

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Has Service

   AAV1

   Insert

   iGABASnFR
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-3z/601

   Plasmid

   #26656

   Depositor

   Jonathan Widom

   Article

   Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

   Insert

   
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-IRES-mCherry FP

    Plasmid

    #52114

    Purpose

    a bicistronic IRES-mCherry containing retroviral vector

    Depositor

    Dario Vignali

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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