We narrowed to 2,185 results for: D10

1. pJT175_GalL_A3A(Y130F)Δ186-BE3

   Plasmid

   #145124

   Purpose

   Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)Δ186-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(Y130F; 1-186AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. peSCBE3-NG

   Plasmid

   #218163

   Purpose

   This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCBE3-NG

   Plasmid

   #218159

   Purpose

   This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / L1111…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-CAGGS-dCas9-KRAB-MeCP2

   Plasmid

   #110824

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas12Max-ABE

   Plasmid

   #195335

   Purpose

   vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-ABE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas12Max-CBE

   Plasmid

   #195337

   Purpose

   vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-CBE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LAMP-mCherry-CRY2

   Plasmid

   #102249

   Purpose

   Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK mKate2-OGT K842M (Puro)

   Plasmid

   #154291

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   K842M mutation (Catalytic Inactive)

   Promoter

   PGK

   Available since

   Dec. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. gGBEv6.3-SpCas9

   Plasmid

   #202629

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpCas9(D10A)-MPGv6.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

   Promoter

   hU6, EF1a, CBh

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCh-KIF5A*-strep

    Plasmid

    #120163

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5A (Kif5a Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5A: 1-559 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid

    #207788

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189922

    Purpose

    AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SaCas9 D10A

    Promoter

    EFS

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-FNLS-P2A-GFP-PGK-Puro

    Plasmid

    #110869

    Purpose

    Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. gGBEv6.3-SpG

    Plasmid

    #202630

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpG(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CMV-ABE8e_dNRCH-BlastR

    Plasmid

    #232720

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    ABE8e-dCas9(NRCH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within Cas9 D10A H840A

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid

    #187953

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187956

    Purpose

    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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