165,688 results

1. Ubi:ccdb-3xHA + mcherry

   Plasmid

   #233302

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-3xHA + mcherry
   

   Use

   Gateway destination

   Tags

   3xHA

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ubi:ccdb-eGFP + mcherry

   Plasmid

   #233300

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-eGFP + mcherry
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

   Plasmid

   #230360

   Purpose

   AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgPPP1R2

   Plasmid

   #227391

   Purpose

   Adhesion module, guide RNA recognizes PPP1R2

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   guide RNA targeting PPP1R2
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-yhaM-RFP

   Plasmid

   #207651

   Purpose

   Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

   Depositor

   Benjamin Woolston

   Article

   Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

   Insert

   yhaM
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUdOm7.1

   Plasmid

   #210287

   Purpose

   Empty backbone with zeocin resistance for high expression in mammalian cells

   Depositor

   Corrie daCosta

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRS-dCas9

   Plasmid

   #220193

   Purpose

   mini-Tn7 dCas9 expression vector

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   promoterless

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046749

   Plasmid

   #145629

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNeonGreen-C1-Nir1-LNS2

    Plasmid

    #220083

    Purpose

    High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX307-VHL-3F-BirA

    Plasmid

    #220147

    Purpose

    Expresses VHL-BirA for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGD-p19

    Plasmid

    #196326

    Purpose

    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    tomato bushy stunt virus p19
    

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXB1-nbMmKappa-Tn5

    Plasmid

    #184286

    Purpose

    Anti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    Ivan Raimondi, New York Genome Center

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbMmKappa-Tn5
    

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #230803

    Purpose

    AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXB1-nbMmIgG2a-Tn5

    Plasmid

    #184288

    Purpose

    Anti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    Ivan Raimondi, New York Genome Center

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbMmIgG2a-Tn5
    

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
    TRAC-3' homology arm (TRAC Human)
    

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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