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We narrowed to 58,334 results for: tra

Showing: 1841 - 1860 of 58334 results

  1. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193317
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193317-plasmid-map-sequence-id-383630

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  2. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Article
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190174-plasmid-map-sequence-id-377202

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  4. pEPQD1CB0911

    Plasmid
    #177067
    Purpose
    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177067-plasmid-map-sequence-id-349529

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  7. pCEP4-SERTR464Q-myc

    Plasmid
    #107468
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R464Q approximately wildtype transport ability
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    R464Q
    Promoter
    CMV
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107468-plasmid-map-sequence-id-205588

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  8. pCEP4-SERTT439M-myc

    Plasmid
    #107466
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    T439M
    Promoter
    CMV
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107466-plasmid-map-sequence-id-205583

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  9. pCEP4-SERTI425L-myc

    Plasmid
    #107465
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425L conferring approximately wildtype transport phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    I425L
    Promoter
    CMV
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107465-plasmid-map-sequence-id-205576

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  12. pJK382

    Plasmid
    #71574
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to asparagine; silent mutat…
    Promoter
    recA
    Available Since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71574-plasmid-map-sequence-id-130942

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Showing: 1841 - 1860 of 58334 results