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We narrowed to 18,759 results for: nls

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Showing: 3721 - 3760 of 18759 results

  1. pMZ-186

    Plasmid
    #195434
    Purpose
    Transient, constitutive expression of synZiFTR: ZF11-p65
    Depositor
    Ahmad Khalil
    Publication
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pUb - 3XFLAG - NLS - ZF11-1 (ZF11) - p65 - RbGpA
    Expression
    Mammalian
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195434-plasmid-map-sequence-id-385010

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  5. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid
    #136660
    Purpose
    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136660-plasmid-map-sequence-id-273363

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  6. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  7. pCMV-hAID-BE3

    Plasmid
    #113417
    Purpose
    Expresses hAID-BE3 in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113417-plasmid-map-sequence-id-228470

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  9. pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop

    Plasmid
    #240258
    Purpose
    Attenuated PROTEUS vector with SRF-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.
    Depositor
    Gregory Neely
    Publication
    Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:
    Insert
    SRF
    Tags
    NLS-VP64
    Expression
    Mammalian
    Mutation
    SRF[aa1-265]; VP64 = 4*VP16[aa437-447]
    Promoter
    SFV subgenomic promoter
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    240258-plasmid-map-sequence-id-495356

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  15. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Publication
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129531-plasmid-map-sequence-id-255632

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  16. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Publication
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

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  18. deltaVP1 + VP2C-GFP-MIOX

    Plasmid
    #166682
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166682-plasmid-map-sequence-id-329930

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  19. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159861
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Publication
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159861-plasmid-map-sequence-id-318036

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  20. vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

    Plasmid
    #160044
    Purpose
    For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)
    Depositor
    Michael Elowitz
    Publication
    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iaaH (iaaH )
    Use
    Synthetic Biology
    Tags
    3xNLS-Citrine
    Expression
    Mammalian
    Promoter
    pEF1s
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    160044-plasmid-map-sequence-id-318469

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  27. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  28. Dendra2-PCNA-19-SV40NLS-4

    Plasmid
    #57735
    Purpose
    Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. mTFP1-PCNA-19-SV40NLS-4

    Plasmid
    #55505
    Purpose
    Localization: Nucleus, Excitation: 462, Emission: 492
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mTFP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139086-plasmid-map-sequence-id-273882

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  37. pT2iC6Dronpa2nlsER4(#636)

    Plasmid
    #184067
    Purpose
    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dronpa2-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184067-plasmid-map-sequence-id-363960

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  40. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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Showing: 3721 - 3760 of 18759 results