-
Plasmid#170982PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ4-PdegV-LacZ
Plasmid#191620PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoterDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8.1-Pcadh-LacZ
Plasmid#191624PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ9-Phcdh-LacZ
Plasmid#191625PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM20-9S-S2 [rpoA]
Plasmid#176217PurposeExpression of TRX-9S-S2 [rpoA]DepositorInsertTRX-9S-S2 [rpoA]
UseSynthetic BiologyTagsHIS and TRXExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM20-9P-S2 [rpoA]
Plasmid#176218PurposeExpression of TRX-9P-S2 [rpoA]DepositorInsertTRX-9P-S2 [rpoA]
UseSynthetic BiologyTagsTRX-HisExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM20-9S-DYW [rpoA]
Plasmid#176220PurposeExpression of TRX-9S-DYW [rpoA]DepositorInsertTRX-9S-DYW [rpoA]
UseSynthetic BiologyTagsTRX-HisExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM20-avi-9S-S2 [rpoA]
Plasmid#176223PurposeExpression of TRX-AviTag-9S-S2 [rpoA]DepositorInsertTRX-Avi-9S-S2 [rpoA]
UseSynthetic BiologyTagsTRX-His-AviTagExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H
Plasmid#187188PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34R, D87RPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H
Plasmid#187184PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32F, L33FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H
Plasmid#187185PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90A, V91APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H
Plasmid#187183PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32A, L33APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox12-PLAT-EGFP-6H
Plasmid#187190PurposeProtein ExpressionDepositorInsertENSDARG00000069463 (alox12 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-PKCgamma-C2-EGFP-6H
Plasmid#187115PurposeProtein ExpressionDepositorInsertENSG00000126583 (PRKCG Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H
Plasmid#187187PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34K, D87KPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90F, V91FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2
Plasmid#161559PurposeInducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM589 EF1α intN-ZF10-NLS-HA in TUPV2
Plasmid#161565PurposeConstitutive expression of intN-ZF10-NLS-HA under the EF1α promoterDepositorInsertintN-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB004 CMV 3xFLAG-NLS-VP16-intN
Plasmid#161549PurposeConstitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoterDepositorInsert3xFLAG-NLS-VP16-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM516 EF1α intC-ZF1-NLS-HA in TUPV2
Plasmid#161551PurposeConstitutive expression of intC-ZF1-NLS-HA under the EF1α promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM522 EF1α intC-ZF10-NLS-HA in TUPV2
Plasmid#161552PurposeConstitutive expression of intC-ZF10-NLS-HA under the EF1α promoterDepositorInsertintC-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-S11-GFP1–9
Plasmid#125666PurposeBinary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertER-S11-GFP1–9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-AM
Plasmid#153427PurposeRetroviral (MSCV) expression of ametrine fluorescent proteinDepositorInsertametrine
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-LO
Plasmid#153428PurposeRetroviral (MSCV) expression of LSSmOrange fluorescent proteinDepositorInsertLSSmOrange
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6d-T
Plasmid#117212PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6d promoter-gRNA
UseSynthetic BiologyTagsExpressionInsectMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTalin2_R7R8
Plasmid#111041PurposePlasmid expresses the talin2 rod domains R7 and R8DepositorInsertTLN2 (Tln2 Mouse)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTalin2_R7
Plasmid#111039PurposePlasmid expresses the talin2 rod domain R7 a 5 helix bundle.DepositorInsertTLN2 (Tln2 Mouse)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 221
Plasmid#98587PurposepMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).DepositorInsertMS2
UseTagsExpressionMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415TEF-NQYFP
Plasmid#99554PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_38J
Plasmid#91154PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt IIDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala13
Plasmid#87174PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proDAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala11
Plasmid#87172PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proAAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala12
Plasmid#87173PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proCAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-Xbp1spliced
Plasmid#80628Purposeexpression of Xbp1 RB isoform in DrosophilaDepositorInsertXbp1 (Xbp1 Fly)
UseTagsExpressionInsectMutationRB isoformPromoterhsp70Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB434
Plasmid#68699PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB433
Plasmid#68698PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB410
Plasmid#68675PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB413
Plasmid#68678PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB233
Plasmid#68624PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12
Plasmid#69942PurposepSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanRDepositorInsertTheoHHAzRaj12,cisRaj12,sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj11
Plasmid#69939PurposepSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanRDepositorInsertTheoHHAzRaj11,cisRaj11,sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKbreak12
Plasmid#69938PurposepSTC2,cisRaj12-sfGFP, KanRDepositorInsertCisRaj12-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq20-T38S38-0X
Plasmid#49522PurposeKanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertconstitutive TetR38
UseTagsExpressionBacterialMutationPromoterPtb38Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
myc-Orai1 E190A pHAGE
Plasmid#22753DepositorInsertOrai1 E190A (ORAI1 Human)
UseLentiviralTagsExpressionMammalianMutationE190APromoterAvailable sinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only