We narrowed to 40,618 results for: TRO

1. pEPQD1CB0863

   Plasmid

   #177081

   Purpose

   MoClo Level 1, position 5, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   secologanin synthase (CrSLS1) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPQD1CB0790

   Plasmid

   #177075

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   iridoid synthase (CrISY) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQD1CB0794

   Plasmid

   #177073

   Purpose

   MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geraniol 8-oxidase (CrG8H) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD1CB0784

   Plasmid

   #177069

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by nos promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   P19 suppressor of gene silencing from Tomato Bushy Stunt Virus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPQD1CB0911

   Plasmid

   #177067

   Purpose

   MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEPQD1CB0776

   Plasmid

   #177066

   Purpose

   MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   strictosidine synthase (CrSTR) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEPQD1CB0774

   Plasmid

   #177064

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEPQD1CB0773

   Plasmid

   #177063

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEPQD1CB0772

   Plasmid

   #177062

   Purpose

   MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   secologanin synthase (CrSLS1) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPQD1CB0771

    Plasmid

    #177061

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    secologanin synthase (CrSLS1) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQD1CB0769

    Plasmid

    #177059

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD1CB0767

    Plasmid

    #177057

    Purpose

    MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD1CB0123

    Plasmid

    #177055

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD1CB0122

    Plasmid

    #177054

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPQD1CB0118

    Plasmid

    #177050

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid synthase (CrISY) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD1CB0116

    Plasmid

    #177048

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD1CB0775

    Plasmid

    #177065

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    strictosidine synthase (CrSTR) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPQD1CB0787

    Plasmid

    #177072

    Purpose

    MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol synthase (CrGES) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD1CB0871

    Plasmid

    #177087

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    secologanin synthase (CrSLS1) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQD1CB0115

    Plasmid

    #177047

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEPQD1CB0114

    Plasmid

    #177046

    Purpose

    MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol 8-oxidase (CrG8H) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEPQD1CB0113

    Plasmid

    #177045

    Purpose

    MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol 8-oxidase (CrG8H) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEPQD1CB0112

    Plasmid

    #177044

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol synthase (CrGES) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEPQD1CB0110

    Plasmid

    #177043

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol synthase (CrGES) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEPQD1CB0104

    Plasmid

    #177038

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    P19 suppressor of gene silencing from Tomato Bushy Stunt Virus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUAP12008

    Plasmid

    #177036

    Purpose

    Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    Cauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEPQD0CM0061

    Plasmid

    #177026

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid oxidase; CYP76A26
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    amplified from cDNA, contains T359S relative to K…

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEPQD0CM0818

    Plasmid

    #177020

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase; geranyl diphosphate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    transit peptide removed

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP

    Plasmid

    #171838

    Purpose

    Mammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    H85E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP

    Plasmid

    #171839

    Purpose

    Mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    K88E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP

    Plasmid

    #171840

    Purpose

    Mammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    I92E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP

    Plasmid

    #171841

    Purpose

    Mammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    C93E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

    Plasmid

    #171842

    Purpose

    Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    R108E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

    Plasmid

    #171843

    Purpose

    Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    R125E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

    Plasmid

    #171844

    Purpose

    Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    K128E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV.U6gLacZ.EFS-NS.H2B-RFP

    Plasmid

    #170363

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. THI ONLY pGAP T-

    Plasmid

    #161503

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

    Plasmid

    #162639

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTOPO-HqERV(del5'-LTR)

    Plasmid

    #164089

    Purpose

    transfection of Hq-ERV without 5'-LTR

    Depositor

    Maik Hintze

    Article

    Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

    Insert

    Hq-ERV
    

    Use

    Eukaryotic expression vector

    Tags

    Expression

    Mutation

    deletion of 5'-LTR

    Promoter

    none (5'-LTR deleted)

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTOPO-HqERV(C1-20)

    Plasmid

    #164090

    Purpose

    transfection of Hq-ERV with light-inducible promoter

    Depositor

    Maik Hintze

    Article

    Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

    Insert

    Hq-ERV
    

    Use

    Eukaryotic expression vector

    Tags

    Expression

    Mutation

    5'-LTR replaced with light-regulatable C1-20…

    Promoter

    light-activatable C1-20

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBz172_CsoS2_N1_GFP

    Plasmid

    #140852

    Purpose

    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 1 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBz175_CsoS2_N4_GFP

    Plasmid

    #140855

    Purpose

    His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 4 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBz173_CsoS2_N2_GFP

    Plasmid

    #140853

    Purpose

    His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBz174_CsoS2_N3_GFP

    Plasmid

    #140854

    Purpose

    His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 3 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLz26_CsoS2_N1-N2_GFP

    Plasmid

    #140851

    Purpose

    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeats 1-2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEBTetBl-CLIP-Centrin3

    Plasmid

    #136855

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEBTetDBl-CLIP-Centrin3

    Plasmid

    #136856

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEBTetDBl-CLIP-FOP

    Plasmid

    #136846

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEBTetDBl-CLIP-POC5

    Plasmid

    #136836

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-POC5 (POC5 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEBTetBl-CLIP-FOP

    Plasmid

    #136845

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge