We narrowed to 18,760 results for: IRE;

1. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90213

   Purpose

   To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Human, Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65

   Plasmid

   #32569

   Depositor

   Sergey Kasparov

   Article

   Benzekhroufa et al Gene Ther. 2009 May;16(5):681-8. Epub 20

   Inserts

   EGFP
   Gal4p65
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   2TPH

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-I-Sce1-eno2:Tau-IRES-egfp

   Plasmid

   #112205

   Purpose

   Used to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoter

   Depositor

   Edward Burton

   Article

   Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

   Insert

   MAPT (MAPT Human)
   

   Use

   Zebrafish expression

   Tags

   eno2 exon 2 translational fusion, ires-egfp at c …

   Expression

   Mutation

   Promoter

   eno2 from zebrafish

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

   Plasmid

   #164777

   Purpose

   cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

   Depositor

   Alexandre Mourot

   Article

   Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

   Insert

   nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E61C

   Promoter

   pGK

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

   Plasmid

   #122991

   Purpose

   Expresses Flag-tagged hRPGRIP1L in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   RPGRIP1L (RPGRIP1L Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177435

   Purpose

   To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.

   Depositor

   David Andrews

   Insert

   Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

   Plasmid

   #157986

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-BLAST Halo-flag-CAD

   Plasmid

   #188117

   Purpose

   Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag and Halo tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-IRES-GFP-TLR4 delta tail

   Plasmid

   #133063

   Purpose

   Retroviral expression of mouse TLR4 with out its cytosolic tail

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   TLR4 delta tail (Tlr4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

    Plasmid

    #103031

    Purpose

    Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-IRES-GFP-CD4-TLR4 cyto

    Plasmid

    #133064

    Purpose

    Retroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD4-TLR4cyto (Tlr4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP

    Plasmid

    #18811

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

15. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

    Plasmid

    #69049

    Purpose

    lentiviral expression of IKZF2 variant 2 with FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2 (IKZF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    CAG

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

    Plasmid

    #131355

    Purpose

    Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Caspase-1 (C285A)

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-Myc-PGK-Puro-IRES-GFP

    Plasmid

    #75124

    Purpose

    Retroviral expression plasmid encoding wild type murine Myc

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177433

    Purpose

    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

    Plasmid

    #204547

    Purpose

    Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deltaUBA

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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