We narrowed to 12,031 results for: cel.2

1. pcDNA3 MDM2 S186D

   Plasmid

   #16235

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ser186 replaced by Asp

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

2. roGFP_iE_C147S_pQE30

   Plasmid

   #48631

   Purpose

   Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   roGFP1
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   insertion E147B, C147S

   Promoter

   Available Since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHis-zfEpoa

   Plasmid

   #65611

   Purpose

   Express soluble His-tagged zebrafish erythropoietin in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

   Insert

   erythropoietin (epoa Zebrafish)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 1199 pAdTrack FKHR AAA

   Plasmid

   #9037

   Depositor

   William Sellers

   Article

   Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

   Insert

   FKHR AAA (FOXO1 Human)
   

   Use

   Adenoviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Promoter

   Available Since

   Sept. 28, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pcdna3 Flag Jip2

   Plasmid

   #59027

   Purpose

   act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

   Depositor

   Roger Davis

   Article

   Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

   Insert

   mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

   Plasmid

   #40731

   Depositor

   Daniel Gerlich

   Article

   Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

   Insert

   Aurora B FRET-sensor
   

   Use

   Tags

   CyPet and YPet

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28b-LanCL2 (6xHis-LanCL2)

   Plasmid

   #73407

   Purpose

   Expresses His tagged LanCL2

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   LanCL2 (LANCL2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. XE122 XDsh delta DIX-GFP-CS2+

   Plasmid

   #16787

   Depositor

   Randall Moon

   Article

   Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

   Insert

   Xdsh-delta DIX (dvl2.L Frog)
   

   Use

   Xenopus expression

   Tags

   gfp

   Expression

   Mutation

   deletion of aa 1-159

   Promoter

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 3540 pMIG Bid p15

   Plasmid

   #8789

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bid p15 (Bid Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3538 pMIG Bad

    Plasmid

    #8787

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bad (Bad Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

12. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

    Plasmid

    #192406

    Purpose

    To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Turbo GFP
    

    Use

    Synthetic Biology

    Tags

    N7

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJTX-GL3

    Plasmid

    #11981

    Purpose

    Reporter gene with mouse c-jun promoter and point mutation of AP1 binding site

    Depositor

    Ron Prywes

    Article

    Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

    Insert

    c-jun promoter (Jun Mouse)
    

    Use

    Luciferase

    Tags

    Luc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

14. p3×FLAG-CMV-14-LanCL2

    Plasmid

    #73391

    Purpose

    Expresses FLAG tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Use

    Tags

    3x FLAG tagged

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJSX-GL3

    Plasmid

    #11980

    Purpose

    Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

    Depositor

    Ron Prywes

    Article

    Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

    Insert

    c-jun promoter (Jun Mouse)
    

    Use

    Luciferase

    Tags

    Luc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pJSTX-GL3

    Plasmid

    #11982

    Purpose

    Reporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites

    Depositor

    Ron Prywes

    Article

    Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

    Insert

    c-jun promoter (Jun Mouse)
    

    Use

    Luciferase

    Tags

    Luc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

    Plasmid

    #192380

    Purpose

    To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcdna3 T7 Jip2

    Plasmid

    #59028

    Purpose

    act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

    Depositor

    Roger Davis

    Article

    Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

    Insert

    MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTC365

    Plasmid

    #91214

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 1130 pBabe puroL HA FKHR H215R AAA

    Plasmid

    #9035

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pSR-siKlf5

    Plasmid

    #41740

    Depositor

    Jonathan Katz

    Article

    Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pRSFDuet-1_VNp-DARPinOFF7

    Plasmid

    #182397

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-4T MDM2 S186D

    Plasmid

    #16239

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser186 replaced by Asp

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pGEX-4T MDM2 S166D

    Plasmid

    #16238

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser166 replaced by Asp

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

26. ERroGFP_C147S_pCDNA3

    Plasmid

    #48630

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S

    Promoter

    Available Since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ERrefGFP1_pCDNA3

    Plasmid

    #48635

    Purpose

    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERrefGFP1
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S,C204Q

    Promoter

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSP64R1 SHP2 D61A

    Plasmid

    #8326

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    D61A (activated mutant)

    Promoter

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

29. pTC363

    Plasmid

    #91211

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. roGFP_iE_C204S_pQE30

    Plasmid

    #48632

    Purpose

    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    roGFP1
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    insertion E147B, c204S

    Promoter

    Available Since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTC303

    Plasmid

    #91210

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. M34 Xdsh PDZ bait-pGBT9

    Plasmid

    #17190

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    Xdsh (dvl2.L Frog)
    

    Use

    Tags

    Gal4-DBD

    Expression

    Yeast

    Mutation

    Dsh bp 343-1191

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

33. pSP64R1 SHP2 E76A

    Plasmid

    #8327

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    E76A (activated mutant)

    Promoter

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

34. XE160 XDshHA-pt mutant PDZ-CS2+

    Plasmid

    #16780

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    Dishevelled (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    HA

    Expression

    Mutation

    point mutation in the binding pocket of the PDZ b…

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

35. XE161 XDshHA-triple pt mutant-CS2+

    Plasmid

    #16779

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    Dishevelled (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    HA

    Expression

    Mutation

    triple point mutation outside of the PDZ binding …

    Promoter

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

36. HOXB4-NA-MSCV

    Plasmid

    #20987

    Depositor

    Corey Largman

    Article

    Krosl et al Oncogene. 1998 Jul 2. 16(26):3403-12.

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N51A (makes DNA binding mutant protein)

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pAGK55

    Plasmid

    #228114

    Purpose

    Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5-TRBP-L326A/V336A/H338A

    Plasmid

    #215894

    Purpose

    Expression of TRBP-L326A/V336A/H338A

    Depositor

    Kumiko Ui-Tei

    Article

    Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

    Insert

    TRBP-L326A/V336A/H338A (TARBP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5-TRBP-V336A/H338A/Y358A

    Plasmid

    #215893

    Purpose

    Expression of TRBP-V336A/H338A/Y358A

    Depositor

    Kumiko Ui-Tei

    Article

    Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

    Insert

    TRBP-V336A/H338A/Y358A (TARBP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J

    Plasmid

    #146690

    Purpose

    Insect Expression of DmHPat_57-968-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-968-dsRNAres (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A1758G and one non silent G56…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J

    Plasmid

    #146695

    Purpose

    Insect Expression of DmHPat_57-968-del500-684

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-968-del500-684 (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAc5.1B-HA-DmHPat_57-968-dsRNAres_H

    Plasmid

    #146464

    Purpose

    Insect Expression of DmHPat_57-968-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-968-dsRNAres (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation T477C and one non silent muta…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192399

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192398

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

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    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

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    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge