Skip to main content
Addgene
Showing: 1901 - 1920 of 2316 results
  1. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Expression
    Mutation
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pMDC32B-AtMIR390a-B/c

    Plasmid
    #51776
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Use
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pENTR-AtMIR390a-B/c

    Plasmid
    #51778
    Purpose
    Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    Insert
    AtMIR390a-B/c
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. CRISPseq-BFP-backbone

    Plasmid
    #85707
    Purpose
    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid
    #213965
    Purpose
    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pMDC123SB-AtMIR390a-B/c

    Plasmid
    #51775
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    Insert
    AtMIR390a-B/c
    Use
    Plant expression
    Tags
    Expression
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Promoter
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1901 - 1920 of 2316 results