We narrowed to 15,566 results for: LEA

1. ΔCT163-GFP

   Plasmid

   #33116

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the last 163 aa of the variable cytop…

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. ΔCT183-GFP

   Plasmid

   #33117

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the last 183 aa of the variable cytop…

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

3. VCDstubΔ741-752-GFP

   Plasmid

   #33123

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains a signal sequence fused to a very small …

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREDTAI

   Plasmid

   #51627

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

   Plasmid

   #86413

   Purpose

   nuclear split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   Nop1pr-GFP11-mCherry-PUS1
   

   Use

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   NOP1

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

   Plasmid

   #86414

   Purpose

   nuclear split GFP11 reporter without mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-PUS1
   

   Use

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   NOP1pr

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDN41

   Plasmid

   #61344

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS9 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDN77

   Plasmid

   #61347

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and NES IkBa

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCLASP_mScarlet_CLASP_RGS2membrane

    Plasmid

    #133086

    Purpose

    Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    mScarlet-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NET31 mRFP-N2 (490)

    Plasmid

    #61984

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

    Insert

    NET31 (TMEM209 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCL1161

    Plasmid

    #217964

    Purpose

    For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP, APEX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E174R, R951K, R955A

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T7-RelA

    Plasmid

    #21984

    Depositor

    Warner Greene

    Article

    Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

    Insert

    NFkB p65 subunit (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    For more details for this expression construct,…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

17. NET33 pEGFP-N2 (597)

    Plasmid

    #61989

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREDIA

    Plasmid

    #51625

    Purpose

    E. coli genome modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRFP-NLS

    Plasmid

    #105296

    Purpose

    To visualize nuclear dynamics in living cells

    Depositor

    Kenneth Yamada

    Article

    Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    aa encoding 172-192

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCL1324

    Plasmid

    #217965

    Purpose

    Bacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Imp5 (IPO5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    plac

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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