We narrowed to 3,857 results for: SPL;

1. pQE80l A-CC-A

   Plasmid

   #196478

   Purpose

   Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   A-CC-A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PEP111E

   Plasmid

   #97397

   Purpose

   binary construct of plastid-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Plastid target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

   Plasmid

   #140226

   Purpose

   Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   Luciferase + crIFNg
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 FLIPE-100u

   Plasmid

   #13543

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

   Plasmid

   #140229

   Purpose

   Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   AntiCRISPR protein AcrVA1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUBC_HA_2xPP7-VP64

   Plasmid

   #68418

   Purpose

   Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   PP7(deltaFG)
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   human UbC

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJYDN3p

   Plasmid

   #162457

   Purpose

   Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-BirA-eCPX-AP

   Plasmid

   #121907

   Purpose

   Expresses BirA and eCPX-AP

   Depositor

   Per Svenningsen

   Article

   Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

   Inserts

   BirA (birA Synthetic)
   Enhanced Circularly Permuted Outer Membrane Protein X
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and pBAD promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U1

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PEP108E

    Plasmid

    #97394

    Purpose

    binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Use

    Tags

    Plasma membrane target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSC-SmBiT

    Plasmid

    #207632

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-SmBiT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. GST-N1IC

    Plasmid

    #47612

    Purpose

    bacterial expression of GST-myc-Notch1 IC

    Depositor

    Urban Lendahl

    Article

    Beatus et al Development. 1999 Sep;126(17):3925-35.

    Insert

    Notch1 intracellular domain (Notch1 Mouse)
    

    Use

    Tags

    GST and myc

    Expression

    Bacterial

    Mutation

    contains amino acids 1753-2531

    Promoter

    tac

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. PEP113E

    Plasmid

    #97399

    Purpose

    binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Peroxisome target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNV-gfp-IRES-MCS

    Plasmid

    #53122

    Purpose

    Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

    Depositor

    Jeanette Hyer

    Article

    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

    Type

    Empty backbone

    Use

    Retroviral; For expression in chicken cells

    Tags

    EGFP-IRES

    Expression

    Mutation

    Promoter

    SNV (spleen necrosis virus)

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBB-II-I-GFP alpha isoform

    Plasmid

    #22146

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

19. RFP-FKBP-U2AF35

    Plasmid

    #187969

    Purpose

    expresses U2AF35 with N-terminal tags RFP and FKBP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    U2AF35 (U2af1 Mouse)
    

    Use

    Cmv promoter

    Tags

    mRFP-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RFP-FKBP-PRPF38A

    Plasmid

    #187968

    Purpose

    expresses PRPF38A with N-terminal tags RFP and FKBP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    Prp38A (PRPF38A Synthetic, Human)
    

    Use

    Cmv promoter

    Tags

    mRFP-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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