We narrowed to 6,466 results for: sas

1. BtARR1 Cys-less

   Plasmid

   #236274

   Purpose

   Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

   Depositor

   Vsevolod Gurevich

   Article

   Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

   Insert

   arrestin-1 (SAG Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C63A, C128S, C143A, inserted GGT codon after star…

   Promoter

   lac

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

   Plasmid

   #215699

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-ArgiNLS-miRFP670

   Plasmid

   #220600

   Purpose

   Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   miRFP670
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-ArgiNLS-mKate2

   Plasmid

   #220599

   Purpose

   Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mKate2
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-ArgiNLS-AausFP1

   Plasmid

   #220595

   Purpose

   Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-FLEX-ArgiNLS-EGFP

   Plasmid

   #220601

   Purpose

   Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLC045 - pL0_CrGLK (CDS1ns)

   Plasmid

   #203904

   Purpose

   MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CrGLK (without a stop codon)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Silent mutation of BpiI or BsaI cut site for MoCl…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLC047 - pL2_p2x35s-CrGLK-tMAS

   Plasmid

   #203906

   Purpose

   Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CrGLK
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Silent mutation of BpiI or BsaI cut site for MoCl…

   Promoter

   CaMV 2x35S

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB278 - pL0_pT16H2 (pro + 5U)

   Plasmid

   #203889

   Purpose

   T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   T16H2 promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation of BpiI or BsaI cut site for MoClo domes…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUN1224 - pL0_pT3R (pro + 5U)

    Plasmid

    #203892

    Purpose

    T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T3R promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUN1215 - pL0_pDAT (pro + 5U)

    Plasmid

    #203895

    Purpose

    DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    DAT promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Drosophila Usp46-V5

    Plasmid

    #217620

    Purpose

    Mammalian expression of V5 tagged Drosophila Usp46

    Depositor

    Ethan Lee

    Article

    Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

    Insert

    USP46 (Usp12-46 Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-cBEST-v2-SF14P

    Plasmid

    #209448

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from SF14P promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-cBEST-v2-kasOP*

    Plasmid

    #209447

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from kasOP* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR

    Plasmid

    #211820

    Purpose

    Lentiviral vector expressing a dead GFP adenine base editing reporter

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    dead GFP reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and SV40 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS-HZ08-pAAVsc.U6-tracrRNA

    Plasmid

    #211816

    Purpose

    AAV vector expressing tracrRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    U6-tracrRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEos3.2trLATm2allL+A

    Plasmid

    #203750

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm2(allL+A) (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas4_1_Δ2_VA

    Plasmid

    #186439

    Purpose

    Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas-mut4_1_2_VA

    Plasmid

    #186441

    Purpose

    Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas-4/1_2_VB

    Plasmid

    #186442

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge